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Un origen somítico común para los progenitores musculares embrionarios y las células satélite
Jérôme Gros1, Marie Manceau, Virginie Thomé
1Laboratoire de Génétique et de Physiologie du Développement, Developmental Biology Institute of Marseille, CNRS UMR 6545, Université de la Méditerranée, Campus de Luminy, case 907, 13288 Marseille Cedex 09, France.
Nature
|April 22, 2005
Resumen
El crecimiento del músculo esquelético depende de los progenitores musculares. Este estudio revela que los progenitores musculares embrionarios y las células satélite adultas se originan en el dermomiotoma, un compartimento somita dorsal.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Biología del músculo esquelético.
- El rastreo del linaje celular traza el linaje celular.
Sus antecedentes:
- El crecimiento del músculo esquelético en embriones y adultos depende de las células progenitoras musculares.
- El origen embrionario preciso de estos cruciales progenitores musculares sigue siendo en gran medida desconocido.
- Comprender el origen del progenitor es clave para el desarrollo y la regeneración muscular.
Objetivo del estudio:
- Para identificar el origen embrionario de los progenitores del músculo esquelético.
- Para determinar si las células madre musculares adultas (células satélite) comparten este origen embrionario.
- Para dilucidar las vías de desarrollo de las células progenitoras musculares.
Principales métodos:
- Electroporación de somites de pollo con proteína fluorescente verde (GFP) para el rastreo del linaje.
- Microscopía confocal de video para rastrear los movimientos celulares durante el desarrollo.
- Experimentos de injerto de pollitos de codorniz para evaluar la contribución celular y la migración.
Principales resultados:
- El dermomiotoma, la parte dorsal de la somita, fue identificado como la fuente de los progenitores musculares.
- Estos progenitores contribuyen al crecimiento del músculo del tronco durante el desarrollo embrionario y fetal.
- El análisis de linaje a largo plazo confirmó que las células satélite se originan de la misma población de dermoyotomas.
Conclusiones:
- Los progenitores musculares embrionarios y las células satélite adultas comparten un origen común en el desarrollo.
- Este origen se remonta al dermomiotoma.
- Establece un origen unificado para los progenitores musculares a lo largo de las etapas de la vida.
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