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Los autoanticuerpos que hidrolizan el ADN son autoanticuerpos.

A M Shuster1, G V Gololobov, O A Kvashuk

  • 1V.A. Engelhardt Institute of Molecular Biology, Academy of Sciences of Russia, Moscow.

Science (New York, N.Y.)
|May 1, 1992
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Los autoanticuerpos en pacientes con enfermedades autoinmunes exhiben actividad de recortado de ADN. Esta actividad hidrolizadora del ADN, identificada como inmunoglobulina M (IgM) e inmunoglobulina G (IgG), difiere de la desoxirribonucleasa (DNase) estándar.

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Área de la Ciencia:

  • Inmunología Inmunología.
  • Biología Molecular Biología Molecular
  • La bioquímica es la bioquímica.

Sus antecedentes:

  • Los pacientes con patologías autoinmunes pueden exhibir actividades séricas únicas.
  • Los autoanticuerpos son actores clave en las enfermedades autoinmunes.
  • La actividad enzimática de los autoanticuerpos no está completamente caracterizada.

Objetivo del estudio:

  • Investigar la presencia y la naturaleza de la actividad de extracción de ADN en el suero de pacientes con enfermedades autoinmunes.
  • Para caracterizar los autoanticuerpos responsables de la hidrólisis del ADN.
  • Para comparar el patrón de degradación del ADN con las desoxirribonucleasas (DNases) conocidas.

Principales métodos:

  • Se analizaron muestras de suero de pacientes con patologías autoinmunes para la actividad de ADN-nicking.
  • Para la purificación se utilizaron cromatografía líquida de afinidad y de alto rendimiento.
  • La inmunoglobulina M (IgM) y la inmunoglobulina G (IgG) fueron identificadas como los componentes activos.
  • Se evaluó la estabilidad al choque ácido y los patrones de degradación del ADN.

Principales resultados:

  • Se detectó una actividad de extracción de ADN en los sueros de los pacientes y se atribuyó a los autoanticuerpos.
  • La actividad purificada correspondía a IgM e IgG, reaccionando con anticuerpos IgG anti-humanos.
  • Estos autoanticuerpos hidrolizantes del ADN demostraron estabilidad al choque ácido.
  • El patrón de degradación del ADN producido por estos autoanticuerpos era distinto de la DNasa I y la DNasa sanguínea.

Conclusiones:

  • Los autoanticuerpos en enfermedades autoinmunes pueden poseer capacidades de hidrólisis del ADN.
  • Los autoanticuerpos identificados son inmunoglobulinas (IgM e IgG) con actividad parecida a la de la nucleasa.
  • Esta actividad es distinta de las DNasas endógenas y exhibe propiedades de estabilidad únicas.