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Membrane Fluidity01:23

Membrane Fluidity

Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.Fatty acids tails of phospholipids can be either saturated or...
Membrane Fluidity01:26

Membrane Fluidity

Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is a relatively...
Fats as Energy Storage Molecules01:06

Fats as Energy Storage Molecules

Triglycerides are a form of long-term energy storage molecules. They are made of glycerol and three fatty acids. To obtain energy from fat, triglycerides must first be broken down by hydrolysis into their two principal components, fatty acids and glycerol. This process, called lipolysis, takes place in the cytoplasm. The resulting fatty acids are oxidized by β-oxidation into acetyl-CoA, which is used by the Krebs cycle. The glycerol that is released from triglycerides after lipolysis directly...
Tight Junctions01:29

Tight Junctions

Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...
Fats as Energy Storage Molecules01:06

Fats as Energy Storage Molecules

Triglycerides are a form of long-term energy storage molecules. They are made of glycerol and three fatty acids. To obtain energy from fat, triglycerides must first be broken down by hydrolysis into their two principal components, fatty acids and glycerol. This process, called lipolysis, takes place in the cytoplasm. The resulting fatty acids are oxidized by β-oxidation into acetyl-CoA, which is used by the Krebs cycle. The glycerol that is released from triglycerides after lipolysis directly...
Lipid Absorption01:24

Lipid Absorption

Dietary triglycerides from chyme in the duodenum are mixed with bile salts produced by the liver to emulsify fats. As a result, large droplets are broken down into smaller ones, increasing the surface area for enzymatic action. Once emulsified, pancreatic lipases hydrolyze the triglycerides into free fatty acids and monoglycerides.
These breakdown products bind with bile salts and lecithin to form micelles, which quickly pass between microvilli to come in close contact with the apical...

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Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology
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Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology

Published on: June 21, 2013

Los liposomas cerrados térmicamente son liposomas cerrados térmicamente.

Wen-Hua Chen1, Steven L Regen

  • 1Department of Chemistry, Lehigh University, Bethlehem, PA 18015, USA.

Journal of the American Chemical Society
|May 5, 2005
PubMed
Resumen

Los liposomas con puerta térmica (TGL) combinan un anfífilo formador de poros con liposomas sensibles a la temperatura. Por encima de 41°C, los TGL liberan solutos atrapados; por debajo de esta temperatura, la liberación se reduce significativamente.

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Área de la Ciencia:

  • Ciencia de los Biomateriales Ciencia de los Biomateriales.
  • Sistemas de suministro de drogas Sistemas de suministro de drogas.
  • Nanotecnología La nanotecnología es la nanotecnología.

Sus antecedentes:

  • Los liposomas compuestos por 1,2-dipalmitoyl-sn-glycero-3-fosfocolina (DPPC) muestran una transición de fase a 41°C.
  • Las anfifilas formadoras de poros pueden desestabilizar las bicapas lipídicas.
  • Los sistemas de liberación controlada son cruciales para las terapias dirigidas.

Objetivo del estudio:

  • Desarrollar y caracterizar los liposomas con puerta térmica (TGL).
  • Para investigar la liberación dependiente de la temperatura de los solutos acuosos de las TGL.
  • Explorar el potencial de las TGL para la administración de agentes terapéuticos.

Principales métodos:

  • Síntesis de un anfífilo formador de poros (compuesto 1) a partir de lisina, ácido cólico y espermina.
  • Preparación de liposomas utilizando DPPC.
  • Incorporación del compuesto 1 en los liposomas de DPPC para formar TGLs.
  • Medición del flujo de eflujo de carboxifluoresceína de las TGL a diferentes temperaturas.

Principales resultados:

  • El Compuesto 1 efectivamente formó poros en los liposomas DPPC por encima de la temperatura de transición de fase de 41°C.
  • Se observó un eflujo significativo de carboxifluoresceína por encima de 41°C.
  • Las tasas de eflujo se redujeron sustancialmente por debajo de 41 ° C, lo que demuestra la liberación regulada por la temperatura.

Conclusiones:

  • Los TGL demuestran una liberación de soluto ajustable y dependiente de la temperatura.
  • El sistema TGL desarrollado es prometedor para aplicaciones de administración de fármacos controladas y dirigidas.
  • Se necesitan más investigaciones para explorar la eficacia in vivo y el potencial terapéutico de las TGL.