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La proteína de unión a TATA es requerida para la transcripción por las tres polimerasas de ARN nuclear en las células

B P Cormack¹, K Struhl

¹Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115.

|May 15, 1992

Resumen

La proteína de unión a TATA (TBP) de la levadura es esencial para toda la transcripción de genes nucleares, incluida la ARN polimerasa I, II y III. La inactivación de TBP detiene rápida y específicamente la transcripción, confirmando su papel directo en la expresión génica.

Área de la Ciencia:

Biología Molecular Biología Molecular
Genética de la levadura Genética de la levadura
Transcripción genética de la transcripción de genes.

Sus antecedentes:

La proteína de unión a TATA (TBP) es un factor de transcripción crucial en los eucariotas.
Su papel preciso en todas las polimerasas de ARN nuclear en la levadura no se ha aclarado completamente.

Objetivo del estudio:

Investigar el requisito de TBP de levadura para la transcripción mediada por las polimerasas de ARN I, II y III.
Para determinar si la TBP es esencial para todos los genes codificados nuclearmente en la levadura.

Principales métodos:

Utilizó derivados de TBP sensibles a la temperatura y proteolíticamente para la inactivación funcional in vivo.
Se evaluó el impacto de la inactivación de TBP en la transcripción de diversos genes, incluidos los de la ARN polimerasa II, III (U6 snRNA, tRNA) y I (ARN ribosomal).

Principales resultados:

La TBP es indispensable para la transcripción de la ARN polimerasa II, independientemente de la presencia de la caja TATA.
La TBP también se requiere para la transcripción mediada por la ARN polimerasa III y la ARN polimerasa I.
La inactivación de TBP condujo a disminuciones rápidas y específicas en la transcripción en todos los promotores probados.

Conclusiones:

La TBP de levadura es un componente fundamental requerido para la transcripción de todos los genes codificados nuclearmente.
Mecanismos moleculares distintos probablemente sustentan el papel de TBP en las maquinarias de transcripción de las tres polimerasas de ARN.

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