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La adición directa de insulina inhibe una Ca2+-ATPasa de alta afinidad en las membranas plasmáticas de adipocitos

H A Pershadsingh, J M McDonald

|October 11, 1979

Resumen

La insulina puede regular el metabolismo celular al afectar directamente los niveles intracelulares de calcio. Los investigadores encontraron que la insulina inhibe una bomba de calcio en las células de grasa de la rata, lo que sugiere un papel directo en la homeostasis del calcio.

Área de la Ciencia:

El metabolismo celular es el metabolismo celular.
La homeostasis del calcio y el calcio.
Endocrinología Endocrinología.

Sus antecedentes:

Se desconoce el mecanismo preciso de la insulina para regular el metabolismo celular.
Las alteraciones en el calcio intracelular están implicadas en los efectos metabólicos de la insulina.
Las células mantienen un fuerte gradiente de calcio a través de la membrana plasmática.

Objetivo del estudio:

Para investigar el papel del calcio intracelular en el mecanismo de acción de la insulina.
Para identificar la base enzimática para la regulación del calcio en las membranas plasmáticas de los adipocitos.
Para determinar si la insulina interactúa directamente con las enzimas reguladoras del calcio.

Principales métodos:

Fracciones subcelulares enriquecidas con membrana plasmática aisladas de adipocitos de rata.
Se analizó la actividad de ATPasa (Ca2+-ATPasa) estimulada por calcio de alta afinidad.
Investigó el efecto de las concentraciones fisiológicas de insulina en la actividad de la Ca2+-ATPasa.

Principales resultados:

Se identificó una Ca2+-ATPasa de alta afinidad en las membranas plasmáticas de los adipocitos de rata.
Se ha demostrado la inhibición específica de esta Ca2+-ATPasa por adición directa de insulina.
El efecto inhibidor de la insulina se produjo a concentraciones fisiológicas.

Conclusiones:

Una Ca2+-ATPasa en las membranas plasmáticas de los adipocitos puede funcionar como una bomba de extrusión de calcio.
La insulina regula directamente esta Ca2+-ATPasa, lo que tiene un impacto en la homeostasis del calcio.
La modulación directa de la homeostasis del calcio es un evento clave potencial en la acción de la insulina.

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