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Las partículas de lipoproteínas son necesarias para la señalización de Hedgehog y Wingless
Daniela Panáková1, Hein Sprong, Eric Marois
1Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse-108, 01307 Dresden, Germany.
Nature
|May 6, 2005
Resumen
Las partículas de lipoproteínas transportan morfógenos modificados por lípidos como Wingless y Hedgehog, cruciales para la señalización del desarrollo. Los niveles reducidos de lipoproteínas afectan a la propagación del morfógeno y al rango de señalización en el desarrollo de la Drosophila.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- La señalización celular de las células.
- Biología Molecular Biología Molecular
Sus antecedentes:
- Las proteínas Wnt y Hedgehog son morfógenos secretados esenciales que controlan el desarrollo.
- Estas proteínas sufren una modificación lipídica, lo que plantea un desafío para el transporte a larga distancia.
- El mecanismo de propagación del morfogénico hidrofóbico no se entiende bien.
Objetivo del estudio:
- Para investigar el mecanismo de transporte de los morfógenos modificados por lípidos.
- Para determinar el papel de las partículas de lipoproteína en la distribución del morfogeno.
- Para dilucidar cómo se propagan las proteínas Wingless y Hedgehog durante el desarrollo.
Principales métodos:
- Copurificación de las proteínas Wingless, Hedgehog y GPI con partículas de lipoproteínas.
- Estudios de co-localización en el epitelio de las alas de Drosophila.
- Análisis del rango de señalización del morfógeno en larvas con niveles reducidos de lipoproteínas.
Principales resultados:
- Las proteínas sin alas, erizo y GPI se asocian con las partículas de lipoproteína.
- Las partículas de lipoproteínas y estas proteínas co-localizan en el ala de la Drosophila.
- Los niveles reducidos de lipoproteínas conducen a la acumulación de Hedgehog y al estrechamiento del rango de señalización.
- El rango de señalización sin alas también se reduce en larvas con deficiencia de lipoproteínas.
Conclusiones:
- Las partículas de lipoproteína sirven como nuevos vehículos para el transporte de los morfógenos ligados a los lípidos.
- Este mecanismo explica la propagación a largo alcance de las especies sin alas y erizo.
- El transporte mediado por lipoproteínas es crítico para un patrón de desarrollo adecuado.
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