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Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration
Published on: May 19, 2016
El movimiento nuclear regulado por el flujo de Cdc42, MRCK, miosina y actina establece la polarización MTOC en las
Edgar R Gomes1, Shantanu Jani, Gregg G Gundersen
1Department of Anatomy and Cell Biology, Columbia University, New York, NY 10032, USA.
Cell
|May 11, 2005
Resumen
La migración celular implica una reorientación del centro organizador de microtúbulos (MTOC, por sus siglas en inglés). Este estudio revela que el núcleo se aleja del borde celular, reposicionando el MTOC y guiando la migración celular.
Área de la Ciencia:
- Biología celular Biología celular.
- Dinámica del citoesqueleto Dinámica del citoesqueleto
- Migración celular La migración celular es la migración celular.
Sus antecedentes:
- La reorientación del centro de organización de microtúbulos (MTOC) es crucial para la migración celular direccional.
- Los modelos anteriores proponían el movimiento del MTOC durante la reorientación.
Objetivo del estudio:
- Investigar el mecanismo de la reorientación del MTOC en los fibroblastos migratorios.
- Determinar las funciones del posicionamiento nuclear y los componentes del citoesqueleto en la reorientación del MTOC.
Principales métodos:
- Imágenes directas de células vivas de fibroblastos en el borde de la herida.
- Estimulación de la reorientación del MTOC utilizando factores solubles.
- Perturbación de las vías moleculares específicas (Cdc42, miosina, actina, dinina, Par6, PKCzeta).
Principales resultados:
- El núcleo se aleja del borde delantero, mientras que el MTOC permanece estacionario durante la reorientación.
- El movimiento nuclear hacia atrás está vinculado al flujo retrógrado de actina y está regulado por Cdc42, MRCK, miosina y actina.
- Dynein, Par6 y PKCzeta no son necesarios para el movimiento nuclear, pero son esenciales para mantener la posición MTOC en el centroide de la célula.
Conclusiones:
- El reposicionamiento nuclear precede y facilita la reorientación del MTOC en las células migratorias.
- El posicionamiento nuclear y el MTOC están regulados por distintas vías moleculares.
- Este estudio redefine los eventos iniciales en la polarización celular durante la migración.
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