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El factor de transcripción Mesp2 establece fronteras segmentales mediante la supresión de la actividad de Notch
Mitsuru Morimoto1, Yu Takahashi, Maho Endo
1Division of Mammalian Development, National Institute of Genetics, Mishima, Japan.
Nature
|May 20, 2005
Resumen
La segmentación de los vertebrados se basa en las somitas. Este estudio revela que la actividad de Notch oscila en el mesodermo presomítico posterior y se detiene en el anterior, formando límites somíticos a través de Mesp2 y Lfng.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- La embriogénesis es la embriogénesis.
- Biología molecular La biología molecular.
Sus antecedentes:
- La segmentación de los vertebrados implica la formación de somitas.
- El modelo de "reloj y frente de onda" explica la periodicidad somita a través de las oscilaciones de señalización de Notch.
- Falta evidencia directa de la oscilación de Notch y su traducción a patrones segmentarios.
Objetivo del estudio:
- Para visualizar la dinámica de actividad endógena de Notch1 durante la embriogénesis del ratón.
- Para dilucidar el mecanismo de la formación de la frontera somita.
- Para investigar el papel de Mesp2 y Lfng en la traducción de las oscilaciones de Notch.
Principales métodos:
- Visualización in vivo de la actividad endógena de Notch1 en ratones.
- Manipulación genética de los componentes de señalización de Notch.
- Análisis bioquímico de las interacciones genéticas.
Principales resultados:
- La actividad de Notch1 oscila en el mesodermo presomítico posterior (PSM).
- La actividad de Notch1 se detiene en el PSM anterior, formando el frente de onda.
- Los límites de somites se forman en la interfaz de los dominios activados y reprimidos de Notch1.
- Mesp2 suprime la actividad de Notch al inducir Lfng, creando esta interfaz.
Conclusiones:
- El estudio proporciona evidencia directa de la actividad de Notch oscilante y detenida durante la formación de somitas.
- El Lfng inducido por Mesp2 media la traducción de las oscilaciones temporales de Notch en límites espaciales de somitas.
- Este mecanismo aclara cómo el modelo de "reloj y frente de onda" genera estructuras metaméricas en vertebrados.
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