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Updated: Jun 25, 2026

Measuring In Vitro ATPase Activity for Enzymatic Characterization
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Published on: August 23, 2016

La catálisis de la fosfatasa alcalina es ultrasensible a las cargas secuestradas entre los iones de zinc del sitio

Ivana Nikolic-Hughes1, Patrick J O'brien, Daniel Herschlag

  • 1Department of Chemical Engineering, Stanford University, Stanford, California 94305, USA.

Journal of the American Chemical Society
|June 30, 2005
PubMed
Resumen

La fosfatasa alcalina (AP) de Escherichia coli utiliza un sitio activo bimetálico para catalizar las reacciones. Su eficiencia catalítica se correlaciona fuertemente con la carga del sustrato, lo que indica un entorno electrostático altamente sensible.

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