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High-throughput Gene Tagging in Trypanosoma brucei
Published on: August 12, 2016
Genómica comparativa de los protozoos parásitos de las tripanosomatidas
Najib M El-Sayed1, Peter J Myler, Gaëlle Blandin
1Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA. nelsayed@tigr.org
Science (New York, N.Y.)
|July 16, 2005
Resumen
La genómica comparativa de Trypanosoma brucei, Trypanosoma cruzi y Leishmania major revela un proteoma central conservado y expansiones genéticas específicas de la especie. Estos protozoos parásitos no comparten un antepasado endosimbiótico fotosintético.
Área de la Ciencia:
- La genómica es la genómica.
- Parasitología Parasitología.
- Biología Molecular Biología Molecular
Sus antecedentes:
- Trypanosoma brucei, Trypanosoma cruzi y Leishmania major son parásitos protozoarios relacionados y responsables de enfermedades humanas significativas.
- Estos parásitos exhiben distintos ciclos de vida y patologías de enfermedades, lo que sugiere diferencias genómicas subyacentes.
Objetivo del estudio:
- Para comparar el contenido de genes y la arquitectura del genoma de T. brucei, T. cruzi y L. major.
- Identificar las características genómicas conservadas y específicas de la especie.
- Para investigar la historia evolutiva, incluyendo la presencia de endosimbiontes.
Principales métodos:
- Análisis genómico comparativo de T. brucei, T. cruzi y L. major.
- Identificación y caracterización de grupos de genes, regiones sinténicas y genes específicos de la especie.
- Análisis de retroelementos, ARN estructurales y expansiones de la familia de genes.
Principales resultados:
- Se identificó un proteoma central conservado de aproximadamente 6200 genes en grandes grupos de genes sintéticos y policistrónicos.
- Los genes específicos de la especie, particularmente las familias de antígenos de superficie, se encuentran en regiones no sintéticas, internas y subteloméricas.
- La configuración genómica implica retroelementos, ARN estructurales, expansión de la familia de genes, divergencia, adquisición / pérdida y reordenamientos, a menudo asociados con discontinuidades sintéticas.
- No hay evidencia que apoye un antepasado con un endosimbionte fotosintético.
Conclusiones:
- Los genomas de estos trypanosomatids se caracterizan por un núcleo conservado y amplias adaptaciones específicas de la especie.
- La arquitectura genómica refleja los procesos evolutivos que dan forma a la diversidad y patogenicidad de los parásitos.
- Los hallazgos refutan la hipótesis de un antepasado endosimbiótico fotosintético en estos parásitos.
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