Video Experimental Relacionado
Updated: Jun 12, 2026
11:20
Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology
Published on: March 21, 2018
Secuencias de ácido nucleico de virus de primates tipo C en tejidos humanos normales y neoplásicos
1Department of Pathology, University of Chicago, Illinois 60637, USA.
Nature
|May 12, 1977
Resumen
Los fibroblastos pulmonares embrionarios humanos liberan un virus tipo C, HEL-12, inmunológicamente relacionado con los virus de los primates. La evidencia sugiere que este virus no es un contaminante, pero puede estar relacionado con cánceres humanos.
Área de la Ciencia:
- Virología Virología.
- Oncología Oncología.
- Inmunología Inmunología.
Sus antecedentes:
- Los virus tipo C aislados de células humanas generan controversia debido a sus similitudes con los virus tipo C de primates.
- Los hallazgos anteriores de virus tipo C en células humanas a menudo se descartaron como contaminación, a pesar de los repetidos aislamientos.
Objetivo del estudio:
- Para demostrar que el virus HEL-12 tipo C, aislado de los fibroblastos pulmonares embrionarios humanos, no es un contaminante accidental.
- Para investigar el vínculo potencial entre el virus HEL-12 y las enfermedades humanas, incluida la leucemia y el lupus eritematoso sistémico.
Principales métodos:
- Análisis inmunológicos de reactividad cruzada entre los antígenos del virus HEL-12 y los tejidos humanos.
- Detección de inmunoglobulinas inhibidoras específicas contra las transcriptasas inversas en pacientes con leucemia mielógena.
- Análisis de los complejos inmunes glomerulares en pacientes con lupus eritematoso sistémico para los antígenos relacionados con HEL-12.
- Técnicas de hibridación molecular para detectar secuencias de ADN provirales en células HEL-12 y secuencias de ARN homólogas en el ADN de pacientes con cáncer.
Principales resultados:
- Las inmunoglobulinas circulantes en pacientes con leucemia mielógena inhibieron HEL-12 y las transcriptasas inversas del virus tipo C de primates.
- Los complejos inmunes glomerulares de pacientes con lupus eritematoso sistémico contenían antígenos relacionados con HEL-12.
- Se detectaron secuencias de ADN provirales en las células HEL-12 antes de la expresión de antígeno detectable o la liberación del virus.
- Se encontraron secuencias de ácido nucleico homólogas al ARN viral HEL-12 en el ADN de ciertos pacientes con cáncer.
Conclusiones:
- El virus HEL-12 es probablemente un retrovirus humano endógeno, no un contaminante.
- La presencia de antígenos relacionados con HEL-12 y secuencias de ácido nucleico en tejidos humanos y cánceres sugiere un papel potencial en enfermedades humanas.
Videos de Conceptos Relacionados
02:21Cancers Originate from Somatic Mutations in a Single Cell
Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
01:05Cancer-Critical Genes II: Tumor Suppressor Genes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
01:03Rous Sarcoma Virus (RSV) and Cancer
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
02:21Cancers Originate from Somatic Mutations in a Single Cell
Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
01:05Cancer-Critical Genes II: Tumor Suppressor Genes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
01:03Rous Sarcoma Virus (RSV) and Cancer
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...