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Los endosomas asimétricos de Rab 11 regulan el reciclaje delta y especifican el destino celular en el sistema
Gregory Emery1, Andrea Hutterer, Daniela Berdnik
1Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Dr. Bohr Gasse 3-5, 1030 Vienna, Austria.
Cell
|September 3, 2005
Resumen
Un nuevo mecanismo de cell-fate en Drosophila utiliza endosomas de reciclaje para controlar la señalización Notch / Delta, independiente de Numb y Neuralized. Esta vía regula el destino del desarrollo al influir en la formación del compartimento endocítico.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología del desarrollo Biología del desarrollo.
- Biología Molecular Biología Molecular
Sus antecedentes:
- Las células precursoras de órganos sensoriales (SOP) de Drosophila exhiben una división celular asimétrica.
- Numb y Neuralized generalmente regulan el destino celular al influir en la señalización Notch / Delta en las células hijas.
Objetivo del estudio:
- Para identificar los mecanismos independientes de Numb y Neuralized en la especificación del cell-fate.
- Investigar el papel del tráfico endocítico en la regulación del destino celular durante el desarrollo de la Drosophila.
Principales métodos:
- Utilizó Drosophila melanogaster como organismo modelo.
- Se emplean sistemas de cultivo celular (incluidos los de mamíferos) para estudiar las vías endocíticas.
- Investigó la localización e interacciones de las proteínas, centrándose en Rab 11 y las consecuencias nucleares.
Principales resultados:
- Descubrió un nuevo mecanismo para la especificación del cell-fate independiente de Numb y Neuralized.
- Se ha demostrado el tráfico diferencial del ligando Notch Delta a través del reciclaje de endosomas (Rab 11-positivo) en células hijas.
- Se demostró que la falta de formación de endosomas de reciclaje en una célula hija (pIIa) se debe a una reducción en el reclutamiento de la lluvia nuclear en el centrosoma.
- Confirmó el papel esencial del reciclaje de endosomas en la actividad del Delta utilizando cultivos celulares de mamíferos.
Conclusiones:
- El destino celular puede determinarse mediante la regulación de la formación de compartimentos endocíticos específicos.
- La formación de endosomas de reciclaje es fundamental para la actividad de la vía de señalización Notch/Delta.
- Este estudio revela una nueva capa de regulación en la división celular asimétrica y la señalización del desarrollo.
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