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Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
Published on: June 15, 2016
El paisaje transcripcional del genoma de los mamíferos
Science (New York, N.Y.)
|September 6, 2005
Resumen
Los investigadores mapearon las transcripciones del genoma del ratón, identificando más de 16.000 nuevas secuencias codificadoras de proteínas y revelando complejos patrones de transcripción. Esto proporciona una base para estudiar la regulación génica de los mamíferos durante el desarrollo.
Área de la Ciencia:
- La genómica es la genómica.
- Biología Molecular Biología Molecular
- La transcriptómica es el campo de la transcripción.
Sus antecedentes:
- Comprender el complemento completo de las transcripciones codificantes de proteínas es crucial para descifrar la función y la regulación de los genes.
- Estudios anteriores han proporcionado vistas parciales del transcriptoma de ratón, lo que requiere un análisis más completo.
Objetivo del estudio:
- Para mapear de manera exhaustiva los sitios de inicio y terminación de la transcripción en el genoma del ratón.
- Para identificar ADNs complementarios de longitud completa previamente desconocidos (ADNc) y analizar la variación de transcripción.
- Establecer un conjunto de datos fundamentales para el análisis comparativo de la regulación transcripcional de los mamíferos.
Principales métodos:
- Encuesta exhaustiva de los sitios de inicio y terminación de la transcripción.
- Análisis de ADN complementario de longitud completa (ADNc) del genoma del ratón.
- Mapeo genómico de transcripciones identificadas.
Principales resultados:
- Identificación de los límites 5' y 3' para 181.047 transcripciones.
- Descubrimiento de 16.247 nuevas transcripciones codificadoras de proteínas de ratón, incluidas 5.154 que codifican nuevas proteínas.
- Observación de una amplia variación de transcripción debido al uso de promotores alternativos, el empalme y la poliadenilación.
- El mapeo genómico reveló "bosques transcripcionales" con transcripción superpuesta y "desiertos" de baja actividad.
Conclusiones:
- El estudio proporciona una visión completa sin precedentes del transcriptoma de ratón.
- Las nuevas transcripciones identificadas y los conocimientos regulatorios ofrecen nuevas vías para la investigación sobre el desarrollo y la diferenciación de los mamíferos.
- Este conjunto de datos sirve como una plataforma crítica para futuros estudios comparativos de genómica y transcriptómica.
Videos de Conceptos Relacionados
01:10Transcription
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
02:16Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
02:35Chromatin Position Affects Gene Expression
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the timing and level of...
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the timing and level of...
02:16Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
01:17Transcription
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
01:30General Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...