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La transcripción antisense en el transcriptoma de mamíferos
S Katayama1, Y Tomaru, T Kasukawa
1Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Centre (GSC), RIKEN Yokohama Institute, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045, Japan.
Science (New York, N.Y.)
|September 6, 2005
Resumen
La transcripción antisense, el proceso de creación de ARN a partir de la cadena de ADN opuesta, juega un papel importante en la regulación de la expresión génica. Este estudio demuestra que alterar los niveles de ARN antisentido impacta la expresión de ARN mensajero de los sentidos en los mamíferos.
Área de la Ciencia:
- La genómica es la genómica.
- Biología Molecular Biología Molecular
- Reglamento genético Reglamento genético.
Sus antecedentes:
- La transcripción antisense, que se origina en la hebra de ADN opuesta a los genes codificadores de proteínas, está implicada en la regulación génica.
- Las funciones conocidas incluyen la degradación de transcripción mediada por interferencia de ARN y el silenciamiento de genes a nivel de cromatina.
- Los análisis globales del transcriptoma sugieren una transcripción bidireccional generalizada en todo el genoma.
Objetivo del estudio:
- Investigar la importancia funcional de la transcripción antisentido en la regulación génica de los mamíferos.
- Para explorar la relación entre el sentido y la expresión de la transcripción antisentido.
- Proporcionar evidencia experimental para el papel regulador de los ARN antisense.
Principales métodos:
- Análisis global del transcriptoma para identificar y cuantificar las transcripciones de ambas hebras de ADN.
- Perfilado de expresiones para analizar pares de transcripciones sentido/antisentido.
- Perturbación experimental de los niveles de ARN antisentido para observar los efectos en la expresión del ARN mensajero de los sentidos.
Principales resultados:
- Una parte sustancial del genoma exhibe transcripción bidireccional, produciendo tanto transcripciones de sentido como antisenso.
- Las transcripciones antisense frecuentemente vinculan genes vecinos en unidades transcripcionales coordinadas.
- Se observa comúnmente la regulación concordante de los pares de transcripciones sentido/antisenso.
- La perturbación de los niveles de ARN antisenso alteró de manera demostrable la expresión del ARN mensajero de los sentidos.
Conclusiones:
- La transcripción antisense es un fenómeno genómico muy extendido en los mamíferos.
- Las transcripciones antisense contribuyen a la regulación coordinada de la expresión génica.
- La transcripción antisense representa un mecanismo significativo para el control de la salida transcripcional.
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