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Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death
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Conectado con la muerte: la vía mitocondrial (no expurgada) de la apoptosis
Diana Spierings1, Gavin McStay, Maya Saleh
1La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA.
Science (New York, N.Y.)
|October 8, 2005
Resumen
La vía mitocondrial de la apoptosis se basa en la permeabilización de la membrana externa mitocondrial (MOMP). Las proteínas de la familia Bcl-2 controlan este paso crucial, regulando la muerte celular.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología molecular La biología molecular.
- La bioquímica es la bioquímica.
Sus antecedentes:
- La apoptosis, o muerte celular programada, es esencial para el desarrollo y la homeostasis tisular.
- La vía mitocondrial es una ruta importante para iniciar la apoptosis en los vertebrados.
- La permeabilización de la membrana externa mitocondrial (MOMP) es un evento crítico que libera proteínas que activan las caspasas.
Objetivo del estudio:
- Para ilustrar las vías moleculares que rigen la vía mitocondrial de la apoptosis.
- Para resaltar las funciones reguladoras de las proteínas de la familia Bcl-2 en el control de MOMP.
- Para mapear la red de señalización aguas arriba y aguas abajo de MOMP.
Principales métodos:
- Utilizó la base de datos del mapa de conexiones del entorno de conocimiento de transducción de señales (STKE) de Science.
- Se analizaron y visualizaron las vías de señalización involucradas en la apoptosis.
- Centrado en la regulación de la permeabilización de la membrana externa mitocondrial.
Principales resultados:
- La vía mitocondrial de la apoptosis está controlada principalmente por los eventos "arriba" de la MOMP.
- Las proteínas de la familia Bcl-2 son reguladores clave que median y controlan el MOMP.
- La activación de la caspasa, el evento "abajo", orquesta el desmantelamiento celular.
Conclusiones:
- La regulación de la apoptosis está fuertemente concentrada en el nivel de control MOMP.
- Comprender los reguladores aguas arriba, particularmente las proteínas Bcl-2, es crucial para comprender la señalización apoptótica.
- El Mapa de Conexiones proporciona un valioso recurso para visualizar estos complejos procesos celulares.
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