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Harpin, provocador de la respuesta de hipersensibilidad producida por el patógeno vegetal Erwinia amylovora

Z M Wei¹, R J Laby, C H Zumoff

¹Department of Plant Pathology, Cornell University, Ithaca, NY 14853.

Science (New York, N.Y.)

|July 3, 1992

Resumen

Los científicos aislaron harpina, una proteína de Erwinia amylovora, que desencadena reacciones de defensa de las plantas como la respuesta de hipersensibilidad. El arpín es el arpín.

Área de la Ciencia:

Patología vegetal patología vegetal.
Genética microbiana genética de los microbios.
Biología molecular La biología molecular.

Sus antecedentes:

Erwinia amylovora causa inflamación por fuego en las plantas rosáceas.
La respuesta de hipersensibilidad es un mecanismo de defensa de la planta.
Anteriormente no se había caracterizado una proteína que provocaba esta respuesta.

Objetivo del estudio:

Para aislar y caracterizar la proteína que provoca la respuesta de hipersensibilidad de Erwinia amylovora.
Para identificar y analizar el gen que codifica este elicitor.
Para investigar el papel de este elicitor en la patogenicidad y la defensa de las plantas.

Principales métodos:

Aislamiento y purificación del elicitor de proteínas.
Caracterización bioquímica (pH, estabilidad térmica, peso molecular).
La clonación, secuenciación y mutagénesis de genes (Tn5tac1).
Análisis de patogenicidad en pera.
Ensayos de respuesta de hipersensibilidad en tabaco.

Principales resultados:

Se aisló un elicitor proteico, llamado harpin.
Harpin es ácido, térmicamente estable, de 44 kDa, y está asociado con la envoltura celular.
Harpin indujo el colapso de la lámina de la hoja y aumentó el pH externo en las células del tabaco.
El gen del arpín (hrpN) fue identificado dentro del clúster de genes del hrp.
Los mutantes de hrpN no eran patógenos, no provocaban la respuesta de hipersensibilidad y carecían de producción de arpín.

Conclusiones:

Harpin es un factor de virulencia clave de Erwinia amylovora.
Harpin provoca directamente la respuesta de hipersensibilidad en las plantas.
El gen hrpN es esencial para la producción de arpinos, la patogenicidad y la elicitación de respuestas hipersensibles.

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