Video Experimental Relacionado
Updated: Jul 14, 2026
08:00
Derivation of Cardiac Progenitor Cells from Embryonic Stem Cells
Published on: January 12, 2015
Generación de células ES pluripotentes derivadas de la transferencia nuclear a partir de blastocistos clonados con
Alexander Meissner1, Rudolf Jaenisch
1Whitehead Institute and Department of Biology, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA.
Nature
|October 18, 2005
Resumen
La transferencia nuclear alterada (ANT) crea blastocistos anormales que no pueden implantarse, pero pueden generar células madre embrionarias personalizadas (CEM). Esta investigación valida ANT para la producción de ESCs específicos del paciente para las terapias potenciales.
Área de la Ciencia:
- Biología de la reproducción Biología reproductiva.
- Biología de las células madre Biología de las células madre
- Biología del desarrollo Biología del desarrollo.
Sus antecedentes:
- La derivación de células madre embrionarias (CE) a través de la transferencia nuclear ofrece un potencial terapéutico, pero requiere la destrucción del blastocisto.
- Las células ES personalizadas derivadas de transferencia nuclear (NT-ESC) son prometedoras para el tratamiento de la inmunodeficiencia, con estudios recientes de viabilidad en humanos.
- La transferencia nuclear alterada (ANT) es un método propuesto para generar blastocistos no implantables para la derivación de ESC.
Objetivo del estudio:
- Validar experimentalmente el concepto de transferencia nuclear alterada (ANT) para la generación de células madre embrionarias personalizadas (CEM).
- Para evaluar si los blastocistos genéticamente modificados, incapaces de implantarse, aún pueden producir células madre pluripotentes.
Principales métodos:
- Se utilizó la transferencia nuclear para crear blastocistos de ratón a partir de fibroblastos de donantes que contenían un ARN de horquilla corta (shRNA) dirigido a Cdx2.
- Se realizaron evaluaciones morfológicas e implantacionales de los blastocistos clonados generados.
- Las células madre embrionarias pluripotentes se derivaron de blastocistos explantados en cultivo.
Principales resultados:
- Los blastocistos clonados generados exhibían una morfología anormal y carecían de trofoblasto funcional.
- Estos blastocistos anormales no pudieron implantarse en el útero, lo que confirma su naturaleza no viable.
- A pesar de los defectos de desarrollo, los blastocistos generaron eficientemente células madre embrionarias pluripotentes in vitro.
Conclusiones:
- La transferencia nuclear alterada (ANT, por sus siglas en inglés) produce efectivamente blastocistos no implantables.
- Este enfoque ANT genera con éxito células madre embrionarias personalizadas (ESC) sin las preocupaciones éticas de la destrucción de embriones viables.
- ANT representa una estrategia viable para generar ESCs específicos del paciente para aplicaciones de medicina regenerativa.
Videos de Conceptos Relacionados
00:58Embryonic Stem Cells
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
00:57Embryonic Stem Cells
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
01:14Maintenance of the ES Cell State
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
01:24Methods of Nuclear Reprogramming
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.