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Cholesterol Efflux Assay
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La capacidad del suero para disminuir la actividad celular de la acilCoA:colesterol aciltransferasa predice los

Julio A Chirinos1, Juan P Zambrano, Simon Chakko

  • 1Miller School of Medicine, University of Miami, Miami, FL 33138, USA.

Circulation
|October 19, 2005
PubMed
Resumen

La actividad de eflujo de colesterol sérico, medida por su capacidad para disminuir el colesterol para la esterificación, predice de forma independiente los principales eventos cardiovasculares adversos (MACE) y la muerte en hombres. Este hallazgo ofrece nuevos conocimientos sobre la evaluación del riesgo cardiovascular.

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Área de la Ciencia:

  • Medicina Cardiovascular La medicina cardiovascular es una especialidad de la salud.
  • La bioquímica es la bioquímica.
  • El metabolismo de los lípidos.

Sus antecedentes:

  • Investigó la asociación entre la actividad de eflujo de colesterol sérico y la enfermedad arterial coronaria (CAD).
  • Examinó el vínculo entre el eflujo de colesterol y el riesgo de eventos cardiovasculares adversos importantes (MACE) y muerte.

Objetivo del estudio:

  • Para determinar si la actividad de eflujo de colesterol sérico predice la presencia de CAD angiográfica.
  • Evaluar la asociación de la actividad de eflujo de colesterol con el riesgo de MACE y muerte.

Principales métodos:

  • Estudió a 168 hombres sometidos a una angiografía coronaria.
  • Se midió la actividad de eflujo de colesterol in vitro utilizando suero de pacientes y fibroblastos de piel humana.
  • La actividad de eflujo se define por la capacidad del suero para disminuir el colesterol disponible para la esterificación de la acilCoA:colesterol aciltransferasa (ACAT).
  • Se siguió a los pacientes durante 4,5 años para rastrear el MACE y la muerte.

Principales resultados:

  • Los cambios inducidos por el suero en la actividad de ACAT no se correlacionaron con los niveles de HDL o la presencia de CAD.
  • Los pacientes en el tertil más alto de cambio de actividad de ACAT tenían un riesgo significativamente mayor de MACE (HR, 2.15) y muerte (HR, 2.23).
  • Estas correlaciones se mantuvieron independientes de otros marcadores de riesgo como LDL, HDL y proteína C reactiva.

Conclusiones:

  • La capacidad del suero para agotar el colesterol celular para la esterificación de ACAT es un predictor fuerte e independiente de MACE y muerte.
  • Se especuló que esta actividad involucra eflujo mediado por el transportador de cassette de unión de ATP A1 (ABCA1).
  • Se planteó la hipótesis de que el aumento de las partículas de HDL que influyen en ACAT en el suero se relacionan con la actividad limitada de ABCA1 in vivo.