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Updated: May 7, 2026
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Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
Published on: September 28, 2018
Una nueva proteína transformadora (SHC) con un dominio SH2 está implicada en la transducción de la señal mitogénica
G Pelicci1, L Lanfrancone, F Grignani
1Istituto Clinica Medica I, Policlinico Monteluce, University of Perugia, Italy.
Cell
|July 10, 1992
Resumen
Las proteínas SHC recién descubiertas vinculan los receptores activados del factor de crecimiento con las vías de proliferación celular. La sobreexpresión de SHC en células de ratón indujo un fenotipo transformado y la formación de tumores, destacando su papel en la regulación del crecimiento celular de los mamíferos.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- La señalización celular de las células.
- Oncología Oncología.
Sus antecedentes:
- El gen SHC codifica una familia de proteínas adaptadoras que contienen un dominio de homología Src 2 (SH2).
- Las proteínas SHC están implicadas en las vías de transducción de señales iniciadas por los receptores del factor de crecimiento.
Objetivo del estudio:
- Caracterizar el nuevo gen SHC y sus productos proteicos.
- Investigar el papel de las proteínas SHC en la mediación de señales de los receptores activados del factor de crecimiento.
- Para determinar las consecuencias funcionales de la sobreexpresión de SHC en células de mamíferos.
Principales métodos:
- Cribado de la biblioteca de ADNc usando sondas de ADN SH2.
- Análisis de la mancha occidental utilizando anticuerpos anti-SHC.
- Ensayos de unión in vitro con el dominio SHC SH2 expresado bacterialmente.
- Análisis fenotípico de los fibroblastos NIH 3T3 que sobreexpresan SHC, incluida la formación de tumores en ratones desnudos.
Principales resultados:
- Aislamiento y caracterización del ADNc SHC que codifica las proteínas de 46,8 y 51,7 kDa con un dominio C-terminal SH2.
- Identificación de las proteínas SHC de 46, 52 y 66 kDa en líneas celulares de mamíferos.
- Demostración de que las proteínas SHC complejan con y son fosforiladas por el receptor del factor de crecimiento epidérmico activado (EGFR).
- Confirmación de la asociación con el receptor SHC in vitro.
- La sobreexpresión constitutiva de SHC en las células 3T3 del NIH condujo a la transformación y la formación de tumores in vivo.
Conclusiones:
- Los productos genéticos SHC funcionan como intermediarios cruciales, acoplando los receptores activados del factor de crecimiento a las vías de señalización aguas abajo.
- Las proteínas SHC juegan un papel importante en la regulación de la proliferación celular de mamíferos y la oncogénesis.
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