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Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans
Published on: February 13, 2013
La activación de LIN-12/Notch conduce a una regulación a la baja de Vav mediada por microARN en C. elegans
1Integrated Program in Cellular, Molecular, and Biophysical Studies, Howard Hughes Medical Institute, Columbia University College of Physicians and Surgeons, 701 West 168th Street, Room 720, New York, NY 10032, USA.
Science (New York, N.Y.)
|October 22, 2005
Resumen
Los investigadores descubrieron un bucle de retroalimentación que involucra a LIN-12, mir-61 y VAV-1 en las células precursoras vulvales de Caenorhabditis elegans (VPC). Este bucle mejora la señalización LIN-12 para promover el destino celular de 2 grados durante el desarrollo.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Genética La genética.
- Biología molecular La biología molecular.
Sus antecedentes:
- Las interacciones célula-célula y el cruce de vías de señalización son cruciales para la especificación del destino celular.
- En Caenorhabditis elegans, las células precursoras vulvales (VPC) adoptan destinos distintos (1 grado y 2 grados) basados en los niveles de señalización.
Objetivo del estudio:
- Para identificar nuevos reguladores de la especificación del destino del VPC.
- Para dilucidar los mecanismos moleculares subyacentes al bucle de retroalimentación en la especificación VPC de 2 grados.
Principales métodos:
- Identificación del microARN (mir-61) como objetivo transcripcional directo de LIN-12.
- Análisis del papel del mir-61 en la promoción del destino del VPC de 2 grados.
- Identificación de vav-1 como objetivo de mir-61 y su papel en la regulación descendente de VAV-1.
Principales resultados:
- LIN-12 regula directamente la expresión de mir-61.
- mir-61 promueve el destino de 2 grados de VPC.
- VAV-1 es un objetivo de mir-61, y su regulación a la baja mejora la actividad de LIN-12, reforzando el destino de 2 grados.
Conclusiones:
- Se identifica un bucle de retroalimentación que involucra a LIN-12, mir-61 y VAV-1.
- Este bucle amplifica la señalización LIN-12 para garantizar una especificación robusta del destino VPC de 2 grados.
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The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
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The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
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