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El complejo TCP1 es un acompañante molecular en la biogénesis de la tubulina
M B Yaffe1, G W Farr, D Miklos
1Department of Pharmacology, Case Western Reserve School of Medicine, Cleveland, Ohio 44106.
Nature
|July 16, 1992
Resumen
El polipéptido-1 del complejo T (TCP1) actúa como un acompañante para las proteínas de tubulina recién sintetizadas en el citosol. Esta función de acompañante es crucial para que la tubulina logre la conformación correcta para el ensamblaje de microtúbulos.
Área de la Ciencia:
- El plegamiento de proteínas citosólicas.
- Las chaperonas moleculares son acompañantes.
- La biogénesis de los microtúbulos.
Sus antecedentes:
- El polipéptido complejo T-1 (TCP1) está implicado en el plegamiento de proteínas citosólicas en eucariotas.
- Los objetivos moleculares específicos de TCP1 siguen sin ser identificados.
- La tubulina es esencial para la formación de microtúbulos, pero su plegamiento de subunidad se entiende mal.
Objetivo del estudio:
- Investigar el papel de TCP1 en la biogénesis de las subunidades de alfa y beta tubulina recién traducidas.
- Para identificar las dianas moleculares de TCP1 en el citosol.
- Para dilucidar los cambios conformacionales que la tubulina sufre durante su plegamiento temprano.
Principales métodos:
- Estudió la biogénesis de la tubulina en el lisado de reticulocitos de conejo.
- Se utilizan ensayos de sensibilidad a la proteasa para evaluar la conformación de las proteínas.
- Los complejos de proteínas analizados mediante cromatografía de exclusión de tamaño (complejo 900K).
- Identificaron los principales constituyentes proteicos del complejo a través de la reactividad cruzada de anticuerpos.
Principales resultados:
- Se encontraron subunidades de tubulina recién sintetizadas en un complejo 900K sensible a la proteasa.
- La adición de Mg-ATP liberó subunidades de tubulina en una forma resistente a la proteasa y capaz de ensamblarse.
- El complejo 900K contenía predominantemente una proteína 58K reconocida por los anticuerpos anti-TCP1.
- TCP1 fue identificado como un componente importante del complejo de unión a la tubulina recién traducida.
Conclusiones:
- TCP1 funciona como un acompañante citosólico específicamente para la biogénesis de la tubulina.
- TCP1 facilita la adquisición de una conformación adecuada para el ensamblaje de tubulinas.
- Este estudio identifica a la tubulina como un objetivo molecular de TCP1, aclarando su papel en la maduración de las proteínas del citoesqueleto.
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