Video Experimental Relacionado
Updated: Aug 11, 2026
09:28
Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes
Published on: January 10, 2017
Inmovilización de las proteínas en los nanotubos de nitruro de boro
Chunyi Zhi1, Yoshio Bando, Chengchun Tang
1Advanced Materials Laboratory, National Institute for Materials Science (NIMS), Namiki 1-1, Tsukuba, Ibaraki 305-0044, Japan. zhi.chunyi@nims.go.jp
Journal of the American Chemical Society
|December 8, 2005
Resumen
Las proteínas se pueden inmovilizar directamente en los nanotubos de nitruro de boro (BNNT) debido a su afinidad natural. La funcionalización no covalente de las BNNTs mejora este proceso de inmovilización de proteínas de manera efectiva.
Área de la Ciencia:
- Ciencia de los materiales Ciencia de los materiales.
- Biotecnología La biotecnología es la biotecnología.
- Nanotecnología La nanotecnología es la nanotecnología.
Sus antecedentes:
- La inmovilización de las proteínas es crucial para los biosensores y los biomateriales.
- Los nanotubos de nitruro de boro (BNNT) ofrecen propiedades únicas para aplicaciones biomédicas.
Objetivo del estudio:
- Para investigar la inmovilización directa de proteínas en BNNTs.
- Para determinar las condiciones óptimas para la adsorción de proteínas en BNNTs.
Principales métodos:
- Utilizando BNNTs no covalentemente funcionalizados.
- Empleando microscopía electrónica de transmisión de alta resolución (HRTEM).
- Realización de análisis por espectroscopia dispersiva de energía (EDS).
Principales resultados:
- Se ha demostrado la inmovilización directa de proteínas sin reactivos en BNNTs.
- Se confirmó una afinidad natural entre proteínas y BNNTs.
- Mostró una inmovilización efectiva utilizando BNNTs no covalentemente funcionalizados.
Conclusiones:
- Las proteínas pueden ser efectivamente inmovilizadas en BNNTs sin agentes de acoplamiento.
- La funcionalización no covalente es clave para una óptima adsorción de proteínas en los BNNT.
- Este hallazgo abre nuevas vías para el desarrollo de biosensores y biomateriales basados en BNNT.
Videos de Conceptos Relacionados
01:20Overview of Nitrogen Metabolism
Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of ammonia, ammonium ions, nitrate, nitrite, or nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this nitrogen...
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this nitrogen...
01:13meta-Directing Deactivators: –NO2, –CN, –CHO, –CO2R, –COR, –CO2H
All meta-directing substituents are deactivating groups. These substituents withdraw electrons from the aromatic ring, making the ring less reactive toward electrophilic substitution. For example, the nitration of nitrobenzene is 100,000 times slower than that of benzene because of the deactivating effect of the nitro group. The first step in an electrophilic aromatic substitution is the addition of an electrophile to form a resonance-stabilized carbocation. The energy diagrams for the...
01:17Nondepolarizing (Competitive) Neuromuscular Blockers: Mechanism of Action
Nondepolarizing neuromuscular blockers induce paralysis by competitively blocking nicotinic acetylcholine receptors at the muscle end plate. Examples include pancuronium, mivacurium, vecuronium, and rocuronium. These quaternary ammonium derivatives are administered intravenously, are poorly absorbed, and are excreted via the kidneys.
Competitive antagonists prevent acetylcholine from binding to its receptor, inhibiting membrane depolarization. Without conformational changes or intrinsic...
Competitive antagonists prevent acetylcholine from binding to its receptor, inhibiting membrane depolarization. Without conformational changes or intrinsic...
01:27Nondepolarizing (Competitive) Neuromuscular Blockers: Pharmacological Actions
Nondepolarizing neuromuscular blockers prevent the membrane depolarization of muscle cells and inhibit muscle contraction. These are usually administered with anesthetics to achieve complete muscle relaxation. Upon administration, these drugs first block the small, rapidly contracting muscles of the face and hands, followed by the larger muscles of the trunk and the intercostal muscles. The diaphragm is the last muscle to be affected.
Although all competitive neuromuscular blockers are designed...
Although all competitive neuromuscular blockers are designed...
01:11Nondepolarizing (Competitive) Neuromuscular Blockers: Pharmacokinetics
All neuromuscular blocking agents are injected intravenously because they are poorly absorbed from the GI tract. Rapid onset is achieved with intravenous administration, although absorption is also adequate from an intramuscular injection. Since these agents are highly ionized, they do not readily penetrate cell membranes or cross the blood-brain barrier.
Instead, they are transported by the blood to different tissues. Muscles with a greater blood supply (arteries) and blood flow receive more...
Instead, they are transported by the blood to different tissues. Muscles with a greater blood supply (arteries) and blood flow receive more...
01:26Directly Acting Muscle Relaxants: Dantrolene and Botulinum Toxin
Directly acting muscle relaxants like dantrolene and botulinum toxin (BoNT) have distinct mechanisms and applications. Dantrolene, a hydantoin derivative, acts on the ryanodine receptor (RYR1) in skeletal muscle cells. RYR1 are calcium channels present at the sarcoplasmic reticulum membrane. In response to excitation, they release calcium ions from the sarcoplasmic reticulum to the cytosol. Calcium promotes actin-myosin-mediated contraction of muscles.
The binding of dantrolene to the RYR1...
The binding of dantrolene to the RYR1...