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El mecanismo de la síntesis de primeras de replicación del ADN por la ARN polimerasa

Nikolay Zenkin¹, Tatyana Naryshkina, Konstantin Kuznedelov

¹Waksman Institute, Rutgers University, Piscataway, New Jersey 08854, USA. nicserzen@mail.ru

|February 3, 2006

Resumen

Las polimerasas de ARN especializadas pueden sintetizar los primers de replicación del ADN. Un complejo de transcripción único con un híbrido de ARN-ADN extendido permite la síntesis de primeras para la replicación de bacteriófagos M13.

Área de la Ciencia:

Biología Molecular Biología Molecular
Virología Virología.
La bioquímica es la bioquímica.

Sus antecedentes:

La replicación del ADN generalmente requiere primers de ARN sintetizados por las primasas.
Algunos sistemas utilizan la ARN polimerasa dependiente del ADN para la síntesis de primeras, lo que plantea un desafío para la formación de complejos.
El bacteriófago M13 emplea la ARN polimerasa para la síntesis de primeras de replicación.

Objetivo del estudio:

Para dilucidar el mecanismo de la replicación catalizada por la ARN polimerasa en la síntesis de primeras.
Para investigar la base estructural para la síntesis de primeras por ARN polimerasa.
Para entender cómo el extremo 3' del ARN se hace accesible para la interacción de la ADN polimerasa.

Principales métodos:

Análisis estructural de complejos de transcripción.
Análisis bioquímicos para estudiar la actividad de la ARN polimerasa.
Investigando los mecanismos de replicación de los bacteriófagos M13.

Principales resultados:

Se forma un nuevo complejo de transcripción durante la síntesis del primer del bacteriófago M13.
Este complejo presenta un híbrido de ARN-ADN sobreextenso dentro de la ARN polimerasa.
El híbrido extendido posiciona el extremo 3' del ARN para el compromiso de la ADN polimerasa.

Conclusiones:

La ARN polimerasa puede formar complejos de transcripción únicos para la replicación.
Este mecanismo implica un híbrido de ARN-ADN extendido, lo que facilita la síntesis de primeras.
Mecanismos similares pueden estar involucrados en la replicación y regulación de la transcripción de otros elementos móviles bacterianos.

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