Video Experimental Relacionado
Updated: Jul 8, 2026
13:40
Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms
Published on: July 7, 2011
Una clase de proteínas de membrana que dan forma al retículo endoplasmático tubular
Gia K Voeltz1, William A Prinz, Yoko Shibata
1Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
Cell
|February 14, 2006
Resumen
Los reticulones y DP1/Yop1p son proteínas esenciales de la membrana integral que dan forma al retículo endoplasmático (ER) mediante la estabilización de su red tubular. Su ausencia interrumpe la estructura de ER, destacando su papel en la morfogénesis de la membrana.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología de la Membrana Biología de la Membrana.
- La bioquímica es la bioquímica.
Sus antecedentes:
- La forma tubular característica del retículo endoplasmático (ER) es crucial para su función.
- Los mecanismos que generan y mantienen la red tubular de la ER no se comprenden completamente.
Objetivo del estudio:
- Investigar los mecanismos moleculares subyacentes a la formación y mantenimiento de la red tubular de la ER.
- Para identificar las proteínas clave involucradas en la conformación de los túbulos de la membrana ER.
Principales métodos:
- Utilizó un sistema in vitro para estudiar la formación de la red de ER.
- Investigó el papel de las proteínas de reticulón (Rtn4a/NogoA) y DP1/Yop1p utilizando enfoques bioquímicos y genéticos.
- Examinó la localización y función de proteínas en levadura (S. cerevisiae) y células de mamíferos.
Principales resultados:
- Se requieren proteínas de membrana integral, reticulones (Rtn4a/NogoA) y DP1/Yop1p, para la formación de la red de ER in vitro.
- Estas proteínas están predominantemente localizadas en el ER tubular, excluyéndolas de las láminas de ER.
- La sobreexpresión de los reticulones induce estructuras de membrana tubular.
- La ausencia simultánea de reticulones y Yop1p en la levadura conduce a una ER tubular interrumpida.
Conclusiones:
- Los reticulones y DP1/Yop1p actúan como proteínas "morfogénicas" que estabilizan los túbulos de la membrana ER altamente curvados.
- Estas proteínas son esenciales para generar y mantener la estructura tubular característica del retículo endoplasmático.
Videos de Conceptos Relacionados
01:49Golgi Apparatus
As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are transported by microtubule-based motor proteins and fuse together to form vesicular tubular clusters, subsequently arriving at the Golgi apparatus, a eukaryotic endomembrane organelle that often has a distinctive ribbon-like appearance.The Golgi apparatus is a major sorting and dispatch station for the products of the ER. Newly arriving vesicles enter...
01:09Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
01:43The Endoplasmic Reticulum
The endoplasmic reticulum or ER makes up for more than half of the membranes in a cell and accounts for 10% of total cell volume. It is also the primary protein and lipid synthesis factory for most cell organelles, such as the Golgi apparatus, lysosomes, secretory vesicles, and the plasma membrane. Despite being the most extensive and functionally complex subcellular organelle, ER was the last to be discovered. After years of deliberation, Keith Porter and George Palade in the year 1954,...
01:28Protein Translocation Machinery on the ER Membrane
The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the translocon complex.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the translocon complex.
01:43The Endoplasmic Reticulum
The endoplasmic reticulum or ER makes up for more than half of the membranes in a cell and accounts for 10% of total cell volume. It is also the primary protein and lipid synthesis factory for most cell organelles, such as the Golgi apparatus, lysosomes, secretory vesicles, and the plasma membrane. Despite being the most extensive and functionally complex subcellular organelle, ER was the last to be discovered. After years of deliberation, Keith Porter and George Palade in the year 1954,...
01:09Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...