Jove
Visualize
Contáctanos

Videos de Conceptos Relacionados

Convergent Evolution01:54

Convergent Evolution

Evolution shapes the features of organisms over time, ensuring that they are suited for the environments in which they live. Sometimes, selection pressure leads to the rise of similar but unrelated adaptations in organisms with no recent common ancestors, a process known as convergent evolution.
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Gene Duplication and Divergence02:37

Gene Duplication and Divergence

The seminal work of Ohno in 1970 popularized the idea of gene duplication and divergence. DNA sequence comparison studies reveal that a large portion of the genes in bacteria, archaebacteria, and eukaryotes was  generated by gene duplication and divergence, indicating its critical role in evolution.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are characterized.
Introduction to Enzymes01:22

Introduction to Enzymes

The use of enzymes by humans dates to 7000 BCE. Humans first used enzymes to ferment sugars and produce alcohol without knowing that this was an enzyme-catalyzed reaction. Wilhelm Kuhne coined the term 'enzyme' in 1877 from the Greek words ‘en’ meaning ‘in’ or ‘within’ and ‘zyme’ meaning ‘yeast.’
Most enzymes are proteins that speed up biochemical reactions without being consumed. Enzymes contain one or more active sites that bind the substrates and convert them into products. Many enzymes also...
Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
Evolution of New Traits in Microbes01:24

Evolution of New Traits in Microbes

Microorganisms evolve rapidly due to their large population sizes and short generation times, often exhibiting measurable changes within days under laboratory conditions. Natural selection acts on standing genetic variation, enabling the retention and amplification of beneficial traits that confer fitness advantages in changing environments.Adaptive Pigment Regulation in RhodobacterIn Rhodobacter, a genus of purple non-sulfur bacteria, light-harvesting pigments such as bacteriochlorophyll and...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

A biosynthetic survey of hypocrealean biocontrol fungi.

Nature chemical biology·2026
Same author

Expanding the scope of redox-balance growth coupling techniques with a carbon cofeeding strategy.

bioRxiv : the preprint server for biology·2026
Same author

Leveraging a synthetic biology approach to enhance BCG-mediated expansion of Vγ9Vδ2 T cells.

PloS one·2026
Same author

Quantitative Dissection of Agrobacterium Virulence to Generate a Synthetic Ti Plasmid.

ACS synthetic biology·2026
Same author

Synthetic biology for heterologous expression and engineering of fungal polyketide synthases.

Natural product reports·2026
Same author

Enzymology and Structural Basis of Glycosyltransferases Involved in Saponin C28 Carboxylic Acid <i>O</i>‑d‑Fucosylation.

JACS Au·2025
Same journal

Six ways to put the public at the heart of science and policy.

Nature·2026
Same journal

The complex truth about trust in science.

Nature·2026
Same journal

Have people stopped trusting science? The data tell a surprising story.

Nature·2026
Same journal

How FAIR data are helping to build trust in science.

Nature·2026
Same journal

Scientists should recognize their own political biases to build public trust.

Nature·2026
Same journal

Harmonizing standards and resources for the medical genome.

Nature·2026
Ver todos los artículos relacionados
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Video Experimental Relacionado

Updated: May 11, 2026

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
13:30

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

Evolución divergente diseñada de la función enzimática.

Yasuo Yoshikuni1, Thomas E Ferrin, Jay D Keasling

  • 1UCSF/UCB Joint Graduate Group in Bioengineering, Lawrence Berkeley National Laboratory, Berkeley, California 94710, USA.

Nature
|February 24, 2006
PubMed
Resumen
Este resumen es generado por máquina.

Los investigadores diseñaron nuevas enzimas mediante la comprensión de la plasticidad de las proteínas. Este estudio demuestra el diseño racional de la enzima mediante la modificación de los residuos de plasticidad en las terpenosintasas, creando biocatalizadores específicos y activos.

Más Videos Relacionados

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

Videos de Experimentos Relacionados

Last Updated: May 11, 2026

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
13:30

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

Área de la Ciencia:

  • La bioquímica es la bioquímica.
  • Biología Molecular Biología Molecular
  • Ingeniería de enzimas Ingeniería de enzimas Ingeniería de enzimas

Sus antecedentes:

  • Las proteínas con funciones promiscuas desarrollan una mayor especificidad y actividad a través de pequeños cambios en los aminoácidos (plasticidad).
  • Comprender esta evolución molecular divergente puede guiar el diseño de enzimas más específicas y activas.
  • Los residuos de plasticidad en sitios activos son cruciales para la adaptabilidad funcional.

Objetivo del estudio:

  • Investigar cómo los residuos de plasticidad contribuyen a la evolución molecular.
  • Formular una metodología de diseño racional para la ingeniería de enzimas.
  • Para construir nuevas terpenosintasas con funciones catalíticas alteradas.

Principales métodos:

  • Sondeó residuos de plasticidad en el sitio activo de la sintasa gamma-humuleno promiscuo.
  • Recombinan sistemáticamente los residuos de plasticidad identificados utilizando un modelo matemático.
  • Construyó y caracterizó nuevas terpenosintasas.

Principales resultados:

  • Se han diseñado con éxito siete terpenas sintasas específicas y activas.
  • Estas nuevas enzimas utilizan diferentes vías de reacción para producir distintos productos de sesquiterpenos.
  • Demostró la viabilidad de explotar la evolutividad del andamio de proteínas.

Conclusiones:

  • Los enfoques racionales de diseño de enzimas basados en la plasticidad son efectivos.
  • El estudio proporciona evidencia de la utilidad de la comprensión de la evolución molecular en la ingeniería enzimática.
  • Destaca el potencial para crear enzimas personalizadas con las especificidades y actividades deseadas.