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Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons09:21

Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons

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This protocol describes the cell culturing of human midbrain dopaminergic neurons, followed by immunological staining and the generation of neuronal phenotypic profiles from acquired microscopic high-content images allowing the identification of phenotypic variations due to genetic or chemical...
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Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression10:54

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In Parkinson's Disease (PD), Substantia Nigra (SNc) dopaminergic neurons degenerate, leading to motor dysfunction. Here we report a protocol for culturing ventral midbrain neurons from a mouse expressing eGFP driven by a Tyrosine Hydroxylase (TH) promoter sequence, harvesting individual fluorescent neurons from the cultures, and measuring their transcriptome using...
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Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains08:45

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

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The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of...
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Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice09:35

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This protocol describes two different environmental manipulations and a concurrent brain infusion protocol to study environmentally-induced brain changes underlying adaptive behavior and brain repair in adult...
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Primary Culture of Mouse Dopaminergic Neurons11:58

Primary Culture of Mouse Dopaminergic Neurons

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Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various...
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Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

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Dopamine is distinctly regulated in the midbrain nuclei, which contain the cell bodies and dendrites of the dopamine neurons. Here we describe a dissection and sample-handling approach to maximize results, and thus conclusions and insights, on dopamine regulation in the midbrain nuclei of the substantia nigra (SN) and ventral tegmental area (VTA) in...
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Video Experimental Relacionado

Updated: Jan 7, 2026

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
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Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

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Entrada del virus: sésamo abierto.

Mark Marsh1, Ari Helenius

  • 1Cell Biology Unit, MRC Laboratory for Molecular Cell Biology, and Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom. m.marsh@ucl.ac.uk

Cell
|February 25, 2006
PubMed
Resumen
Este resumen es generado por máquina.

Comprender la replicación viral y las interacciones del huésped es clave para desarrollar estrategias antivirales. Una nueva investigación ilumina cómo los virus entran en las células huésped, revelando nuevos objetivos terapéuticos.

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Área de la Ciencia:

  • Virología Virología.
  • Biología celular Biología celular.
  • Biología de Sistemas Biología de Sistemas.

Sus antecedentes:

  • El desarrollo de estrategias antivirales efectivas requiere un conocimiento detallado de los ciclos de replicación viral y las interacciones del huésped.
  • Los virus animales utilizan diversas vías para entrar en las células huésped, un paso crítico en su ciclo de vida.

Objetivo del estudio:

  • Para dilucidar los complejos mecanismos de la entrada viral en las células huésped.
  • Identificar nuevas dianas terapéuticas para las intervenciones antivirales.

Principales métodos:

  • Técnicas de biología celular técnicas de biología celular.
  • Imágenes de células vivas.
  • La biología de sistemas aborda los enfoques de la biología de sistemas.

Principales resultados:

  • Conocimientos detallados sobre las múltiples vías que los virus animales utilizan para la entrada en la célula huésped.
  • Iluminación de las funciones de la endocitosis y el transporte de vesículas intracelulares en la infección viral.
  • Identificación de nuevos objetivos potenciales para inhibir la replicación viral.

Conclusiones:

  • La comprensión de los mecanismos de entrada viral avanza significativamente nuestro conocimiento de las interacciones huésped-patógeno.
  • Se pueden desarrollar nuevas estrategias terapéuticas dirigiéndose a las primeras etapas de la infección viral, específicamente la entrada viral.
  • La investigación continua en biología celular y biología de sistemas es crucial para combatir las enfermedades virales.