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Regiones relacionadas de unión a la ARN polimerasa en el RAP30/74 humano y en Escherichia coli sigma 70

S McCracken¹, J Greenblatt

¹Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada.

Science (New York, N.Y.)

|August 23, 1991

Resumen

Los factores generales de iniciación de la transcripción como el RAP30/74 se unen a la ARN polimerasa II. La subunidad RAP30 es la subunidad RAP30.

Área de la Ciencia:

Biología Molecular Biología Molecular
La bioquímica es la bioquímica.
Genética La genética.

Sus antecedentes:

RAP30/74 es un factor de iniciación de transcripción general heteromérico.
Interactúa con la ARN polimerasa II de los mamíferos.
La subunidad RAP30 comparte similitud de secuencia con E. coli sigma 70.

Objetivo del estudio:

Para investigar la relación funcional entre RAP30 y E. coli sigma 70.
Para determinar si las regiones de unión a la ARN polimerasa están funcionalmente relacionadas.

Principales métodos:

Ensayos de fosforilación in vitro para evaluar la protección de los residuos de serina.
Ensayos de unión utilizando RAP30/74 humano y E. coli ARN polimerasa.
Pruebas de desplazamiento con E. coli sigma 70.

Principales resultados:

La ARN polimerasa II de mamíferos protegió un residuo de serina en la región relacionada con RAP30 sigma 70 de la fosforilación.
RAP30/74 humano unido al ARN polimerasa de E. coli.
E. coli sigma 70 desplazó al RAP30/74 humano de la ARN polimerasa de E. coli.

Conclusiones:

RAP30 y sigma 70 poseen dominios de unión de ARN polimerasa funcionalmente relacionados.
Esto sugiere mecanismos conservados en la iniciación de la transcripción entre especies.

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