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Regulación redox de una proteína tirosina quinasa en el retículo endoplasmático

A R Bauskin¹, I Alkalay, Y Ben-Neriah

¹Lautenberg Center for General and Tumor Immunology, Hebrew University, Hadassah Medical School, Jerusalem, Israel.

|August 23, 1991

Resumen

La proteína tirosina quinasa Ltk del ratón está inusualmente ubicada en el retículo endoplasmático (ER). Su actividad está regulada por el potencial redox celular, un nuevo mecanismo para las proteínas tirosina-cinasas.

Área de la Ciencia:

Biología celular Biología celular.
Biología Molecular Biología Molecular
La bioquímica es la bioquímica.

Sus antecedentes:

Las proteínas tirosina quinasas (PTK) juegan un papel crucial en la señalización celular.
La función y localización de la proteína tirosina quinasa transmembrana Ltk no se entienden completamente.
Las receptoras de las tirosinacinasas suelen estar localizadas en la membrana plasmática.

Objetivo del estudio:

Para determinar la localización subcelular del ratón Ltk proteína tirosina quinasa.
Investigar los mecanismos reguladores de la actividad de Ltk.

Principales métodos:

La inmunofluorescencia indirecta y la tinción inmunogold en células y linfocitos COS transfectados.
Análisis de endoglucosidasa de Ltk en transfectantes y linfocitos COS.

Principales resultados:

Se encontró que Ltk estaba inusualmente localizado en el retículo endoplasmático (ER).
La actividad de Ltk se ve reforzada por agentes alquilantes y tiol-oxidantes, lo que sugiere una función independiente del ligando.
El Ltk activo existe como multimeros ligados al disulfuro, lo que indica la regulación redox.

Conclusiones:

La actividad catalítica de Ltk en la ER puede estar regulada por el potencial redox celular, un nuevo mecanismo para las PTK.
Esta regulación sensible al redox en la ER podría ser una característica compartida con algunas quinasas receptoras.

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