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Videos de Conceptos Relacionados

RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

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Ectopic transcription due to inherited histone methylation may interfere with the ongoing function of differentiated neurons.

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Video Experimental Relacionado

Updated: May 12, 2026

Chromatin Isolation by RNA Purification (ChIRP)
11:09

Chromatin Isolation by RNA Purification (ChIRP)

Published on: March 25, 2012

Una relación de larga distancia entre RNAi y Polycomb.

Elissa P Lei1, Victor G Corces

  • 1Department of Biology, Johns Hopkins University, Baltimore, Maryland, 21218, USA.

Cell
|March 15, 2006
PubMed
Resumen

El mecanismo de interferencia de ARN (RNAi) es esencial para las interacciones físicas entre los cromosomas. Esto sugiere que el RNAi juega un papel en la organización nuclear de orden superior y la regulación génica.

Área de la Ciencia:

  • Biología Molecular Biología Molecular
  • Genética La genética.
  • La epigenética es la epigenética.

Sus antecedentes:

  • Las vías de interferencia de ARN (RNAi) median el silenciamiento de genes transcripcionales específicos de la secuencia a nivel de la cromatina.
  • El complejo represivo Polycomb (PRC) está involucrado en el silenciamiento genético y la regulación de la cromatina.

Objetivo del estudio:

  • Para investigar el papel de la maquinaria RNAi en las interacciones cromosómicas.
  • Para explorar la conexión entre RNAi, PRC y la organización nuclear.

Principales métodos:

  • Utilizó las vías de interferencia de ARN (RNAi).
  • Estudió el complejo represivo Polycomb (PRC).
  • Investigó las interacciones físicas entre los cromosomas.

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Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
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Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

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RNA-Associated Chromatin DNA-DNA Interaction Method
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RNA-Associated Chromatin DNA-DNA Interaction Method

Published on: April 30, 2026

Videos de Experimentos Relacionados

Last Updated: May 12, 2026

Chromatin Isolation by RNA Purification (ChIRP)
11:09

Chromatin Isolation by RNA Purification (ChIRP)

Published on: March 25, 2012

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
10:53

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

RNA-Associated Chromatin DNA-DNA Interaction Method
11:01

RNA-Associated Chromatin DNA-DNA Interaction Method

Published on: April 30, 2026

Principales resultados:

  • La maquinaria RNAi es necesaria para las interacciones físicas de larga distancia entre cromosomas.
  • Estas interacciones están mediadas por el complejo represivo de Polycomb (PRC).

Conclusiones:

  • La maquinaria RNAi está implicada en la regulación de la organización nuclear de orden superior.
  • El ARNi puede influir en la estructura y función del genoma más allá del silenciamiento genético.