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Una ATPasa de tipo P requerida para la enfermedad de los blastos del arroz y la inducción de la resistencia del
Martin J Gilbert1, Christopher R Thornton, Gavin E Wakley
1School of Biosciences, University of Exeter, Washington Singer Laboratories, Perry Road, Exeter EX4 4QG, UK.
Nature
|March 24, 2006
Resumen
Los hongos patógenos de las plantas usan un gen específico, MgAPT2, para la exocitosis durante la infección. Este proceso es crucial para la entrega de proteínas efectoras, suprimiendo las defensas de las plantas y causando enfermedades como la ráfaga de arroz.
Área de la Ciencia:
- Patología Vegetal Patología vegetal.
- Biología Molecular Biología Molecular
- La micología de la micología.
Sus antecedentes:
- Los microorganismos patógenos entregan proteínas a las células vegetales para suprimir las defensas e invadir los tejidos.
- Los mecanismos por los cuales los hongos patógenos de las plantas entregan proteínas durante la infección siguen siendo en gran medida desconocidos.
Objetivo del estudio:
- Para investigar el papel del gen codificante de la ATPasa tipo P, MgAPT2, en el patógeno de la ráfaga de arroz Magnaporthe grisea durante la infección de las plantas.
Principales métodos:
- El reemplazo genético dirigido se utilizó para crear mutantes DeltaMgapt2.
- Se realizaron ensayos de infección en plantas de arroz (hojas y raíces).
- Se realizó un análisis de la secreción extracelular de enzimas y de las estructuras celulares (cisternas tipo Golgi).
Principales resultados:
- MgAPT2 es esencial tanto para la infección foliar como para la raíz por Magnaporthe grisea.
- MgAPT2 es necesario para la rápida inducción de respuestas de defensa del huésped en interacciones incompatibles.
- Los mutantes de DeltaMgapt2 exhiben una disminución en la secreción de enzimas extracelulares y cisternas anormales similares a las de Golgi.
- El crecimiento hifálico y la esporulación no se vieron afectados significativamente por la pérdida de MgAPT2.
Conclusiones:
- El proceso exocitótico dependiente de MgApt2 es crítico para el establecimiento de la enfermedad de la ráfaga del arroz.
- Este estudio revela un nuevo mecanismo de entrega de proteínas empleado por los hongos patógenos de las plantas durante la infección del huésped.
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