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Regulación negativa de una proteína tirosina fosfatasa por la fosforilación de la tirosina
Dirk Schwarzer1, Zhongsen Zhang, Weiping Zheng
1Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Journal of the American Chemical Society
|March 30, 2006
Resumen
La actividad de la proteína tirosina fosfatasa de bajo peso molecular (LMW-PTP) está inhibida por la fosforilación de tirosina en Tyr-131/132.2. Este estudio revela un nuevo mecanismo regulador para LMW-PTP, que afecta la señalización celular y la adhesión.
Área de la Ciencia:
- La bioquímica es la bioquímica.
- La señalización celular de las células.
- Enzimología Enzimología.
Sus antecedentes:
- La proteína tirosina fosfatasa de bajo peso molecular (LMW-PTP) es crucial para la señalización celular.
- Anteriormente se propuso la fosforilación de tirosina en Tyr-131 y Tyr-132 para activar LMW-PTP.
- El estudio de la fosforilación por tirosina de las fosfatasas de tirosina es bioquímicamente desafiante debido a la inestabilidad de la modificación.
Objetivo del estudio:
- Para investigar el impacto funcional de la fosforilación de tirosina en la actividad de LMW-PTP.
- Para superar los desafíos en el estudio de las modificaciones de fosforilación de la tirosina inestable.
- Explorar un nuevo mecanismo de regulación para el LMW-PTP.
Principales métodos:
- Se utilizó la ligadura de proteínas expresadas para crear variantes semisintéticas LMW-PTP.
- Incorporó específicamente en el sitio un imitador de la fosfotirosina (Pmp) en Tyr-131 y Tyr-132.
- Se midió la actividad catalítica de LMW-PTP modificado frente a sustratos conocidos.
Principales resultados:
- La LMW-PTP modificada con fosfonatos mostró una actividad reducida (disminución de 10 a 23 veces).
- Se observó una inhibición contra los péptidos de fosfotirosina del receptor PDGF y p190RhoGap.
- Esto demuestra que la fosforilación de la tirosina inhibe la actividad LMW-PTP.
Conclusiones:
- Proporciona la primera evidencia de una tirosina fosfatasa inhibida por la fosforilación de la tirosina.
- Sugiere un nuevo modelo regulatorio para la función LMW-PTP.
- Destaca el papel de LMW-PTP en la regulación de la adhesión celular a través de este mecanismo.
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