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Un receptor ligado a la proteína G para la hormona paratiroidea y el péptido relacionado con la hormona paratiroidea
H Jüppner1, A B Abou-Samra, M Freeman
1Endocrine Unit, Massachusetts General Hospital, Boston 02114.
Science (New York, N.Y.)
|November 25, 1991
Resumen
Los investigadores clonaron un nuevo receptor de péptidos relacionados con la hormona paratiroidea y la hormona paratiroidea (PTH-PTHrP), lo que demuestra su igual afinidad de unión para ambos ligandos y su papel en una nueva familia de receptores de hormonas reguladoras del calcio.
Área de la Ciencia:
- Endocrinología Molecular La Endocrinología Molecular
- Biología de los receptores Biología de los receptores.
- La homeostasis del calcio y el calcio.
Sus antecedentes:
- La hormona paratiroidea (PTH) y el péptido relacionado con la hormona paratiroidea (PTHrP) son reguladores clave del calcio.
- Comprender sus interacciones con los receptores es crucial para la investigación del metabolismo del calcio.
Objetivo del estudio:
- Clonar y caracterizar el ADN complementario (ADNc) que codifica el receptor PTH-PTHrP.
- Investigar las afinidades de unión y las capacidades de señalización del receptor novedoso.
Principales métodos:
- Clonado del ADNc del receptor utilizando la expresión COS-7 y una biblioteca de ADNc de las células renales de la zarigüeya.
- Evaluación de la afinidad de unión de ligando para PTH y PTHrP.
- Medición de la estimulación de la adenilato ciclasa por PTH y PTHrP.
Principales resultados:
- Clonó con éxito un receptor de 585 aminoácidos con siete dominios transmembrana.
- El receptor expresado exhibió la misma afinidad de unión tanto para PTH como para PTHrP.
- Ambos ligandos estimulan de manera equivalente la actividad de la adenilato ciclasa.
Conclusiones:
- El receptor clonado se une y responde tanto a la PTH como a la PTHrP.
- La homología estructural sugiere una nueva familia de receptores ligados a proteínas G para las hormonas reguladoras del calcio, incluido el receptor de la calcitonina.
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