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Published on: September 29, 2016
Transducción de la señal intra-subunitaria por el quimiorreceptor del aspartato
1Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Science (New York, N.Y.)
|December 23, 1991
Resumen
La señalización transmembrana por los receptores de aspartato bacteriano no requiere interacciones de subunidad. La señalización se produce a través de cambios conformacionales dentro de una sola subunidad, no entre dominios adyacentes.
Área de la Ciencia:
- Microbiología Microbiología.
- Biología Molecular Biología Molecular
- La bioquímica es la bioquímica.
Sus antecedentes:
- Los receptores de la membrana celular son cruciales para la transducción de señales y por lo general consisten en múltiples subunidades.
- El receptor de aspartato bacteriano es un modelo clave para el estudio de los mecanismos de señalización transmembrana.
Objetivo del estudio:
- Para investigar el papel de las interacciones de las subunidades en la señalización transmembrana por el receptor de aspartato bacteriano.
- Para determinar si los dominios citoplasmáticos o de transmembrana son esenciales para la transducción de señales.
Principales métodos:
- Construyendo dímeros de receptores con diferentes composiciones de subunidades: de longitud completa, sin dominio citoplasmático, o sin dominio citoplasmático y transmembrana.
- Evaluación de la metilación del dominio citoplasmático en respuesta a la unión del aspartato a través de diferentes construcciones.
Principales resultados:
- La unión al aspartato estimuló la metilación del dominio citoplasmático en las tres construcciones de los receptores.
- La señalización transmembrana se observó incluso cuando los dominios citoplasmáticos o transmembranares estaban ausentes o truncados.
Conclusiones:
- Las interacciones de subunidades entre los dominios citoplasmáticos o transmembrana no son necesarias para la señalización transmembrana en el receptor de aspartato bacteriano.
- La transducción de señales puede ocurrir a través de cambios conformacionales dentro de una sola subunidad de receptor.
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