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Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...

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Updated: Jun 27, 2026

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
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Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR

Published on: July 30, 2011

Una red de interacción proteína-ADN de C. elegans centrada en el gen.

Bart Deplancke1, Arnab Mukhopadhyay, Wanyuan Ao

  • 1Program in Gene Function and Expression and Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, 01605, USA.

Cell
|June 17, 2006
PubMed
Resumen
Este resumen es generado por máquina.

Este estudio mapea las interacciones del factor de transcripción (TF) en C. elegans utilizando un ensayo de levadura un híbrido gene-centrado. Revela una red de interacción proteína-ADN altamente conectada, identificando nuevos TF y proporcionando información sobre la regulación génica.

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In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay
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In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay

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Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
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Published on: July 30, 2011

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Área de la Ciencia:

  • Biología Molecular Biología Molecular
  • La genómica es la genómica.
  • Biología de Sistemas Biología de Sistemas.

Sus antecedentes:

  • Las redes reguladoras de la transcripción gobiernan la expresión génica a través de las interacciones entre los factores de transcripción (TF) y los genes diana.
  • El mapeo de estas interacciones es un desafío en los sistemas de metazoos en comparación con los organismos unicelulares.
  • Los métodos existentes centrados en TF son menos adecuados para organismos multicelulares complejos.

Objetivo del estudio:

  • Mapear sistemáticamente las interacciones entre el factor de transcripción y el gen objetivo en C. elegans utilizando un enfoque centrado en el gen.
  • Construir una red de interacción proteína-ADN (PDI) para el tracto digestivo de C. elegans.
  • Para identificar nuevos TFs y obtener información sobre la regulación génica de los metazoos.

Principales métodos:

  • Se utilizaron ensayos de levadura unhíbrida (Y1H) de alto rendimiento.
  • Empleó una estrategia centrada en los genes centrada en los promotores de genes.
  • Interacciones investigadas entre 72 promotores de genes del tracto digestivo de C. elegans y 117 proteínas.

Principales resultados:

  • Se identificaron 283 interacciones proteína-ADN, formando una red PDI altamente conectada.
  • La red se enriquece con TFs expresados en el tracto digestivo de C. elegans.
  • Proporcionó anotaciones funcionales para TFs no caracterizados anteriormente e identificó diez nuevos TFs putativos.
  • Se generaron pruebas in vivo que apoyan múltiples IDP.

Conclusiones:

  • Un enfoque centrado en los genes es poderoso para diseccionar las redes reguladoras de la transcripción de metazoos.
  • La red PDI construida ofrece información valiosa sobre la expresión génica diferencial a nivel de sistemas.
  • Este estudio amplía la anotación funcional de los TF de C. elegans y revela nuevos componentes regulatorios.