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TRB3 enlaza la E3 ubiquitina ligasa COP1 con el metabolismo de los lípidos
Ling Qi1, Jose E Heredia, Judith Y Altarejos
1Peptide Biology Laboratories and Gene Expression Laboratories, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Science (New York, N.Y.)
|June 24, 2006
Resumen
Durante el ayuno, Tribbles 3 (TRB3) mejora la degradación de los lípidos promoviendo la degradación de la acetil-coenzima A carboxilasa (ACC). Este descubrimiento revela una nueva vía para mantener el equilibrio energético.
Área de la Ciencia:
- La bioquímica es la bioquímica.
- Regulación del metabolismo La regulación del metabolismo
- Biología celular Biología celular.
Sus antecedentes:
- El ayuno activa las catecolaminas para movilizar los lípidos a través de la inactivación de la acetil-coenzima A carboxilasa (ACC).
- ACC es la enzima que limita la velocidad en la síntesis de ácidos grasos, crucial para la homeostasis energética.
Objetivo del estudio:
- Para aclarar una vía paralela que involucra a Tribbles 3 (TRB3) en la regulación del metabolismo de los lípidos durante el ayuno.
- Investigar el mecanismo por el cual TRB3 influye en la actividad del ACC y la lipólisis.
Principales métodos:
- Estudió el papel de TRB3 en el tejido adiposo durante el ayuno.
- Investigó la interacción de TRB3 con la proteína fotomorfógena constitutiva 1 (COP1) y su efecto en la ubiquitinación del ACC.
- Se utilizaron adipocitos con deficiencia de TRB3 y modelos de ratones transgénicos.
Principales resultados:
- La abundancia de TRB3 aumenta durante el ayuno, estimulando la lipólisis promoviendo la degradación del ACC.
- TRB3 facilita la ubiquitinación del ACC a través de la asociación con COP1, una ligasa de ubiquitina E3.
- Los adipocitos con deficiencia de TRB3 muestran un aumento en los niveles de proteína ACC.
- Los ratones transgénicos que sobreexpresan TRB3 en el tejido adiposo están protegidos de la obesidad inducida por la dieta debido a una mayor oxidación de los ácidos grasos.
Conclusiones:
- Una nueva vía en la que la degradación del ACC mediada por TRB3 contribuye a la lipólisis y la homeostasis energética durante el ayuno.
- Las vías de fosforilación y ubiquitinación convergen en el ACC, regulado por TRB3, para controlar el metabolismo de los lípidos.
- TRB3 representa un objetivo potencial para el manejo de la obesidad y los trastornos metabólicos.
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