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RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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Empalme alternativo: nuevos conocimientos de los análisis globales.

Benjamin J Blencowe1

  • 1Banting and Best Department of Medical Research and Department of Molecular and Medical Genetics, Centre for Cellular and Biomolecular Research, Donnelly CCBR Building, University of Toronto, Toronto, ON M5S 3E1, Canada. b.blencowe@utoronto.ca

Cell
|July 15, 2006
PubMed
Resumen

El empalme alternativo genera una diversidad proteómica significativa en los animales. Esta revisión cubre la investigación actual sobre la identificación de las variantes de transcripción, sus roles en la salud y la enfermedad, y su regulación coordinada.

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Área de la Ciencia:

  • Biología Molecular Biología Molecular
  • La genómica es la genómica.
  • La proteómica es la proteómica.

Sus antecedentes:

  • El empalme alternativo es un mecanismo clave para generar diversidad proteómica y funcional en los organismos metazoares.
  • Los análisis de datos de secuencias y microarrays ponen de relieve el papel significativo del empalme alternativo.

Objetivo del estudio:

  • Revisar los avances recientes en la comprensión del empalme alternativo.
  • Establecer el repertorio de variantes transcritas funcionalmente relevantes.
  • Para explorar los roles de estas variantes en la fisiología normal y de la enfermedad.
  • Comprender la coordinación global del empalme alternativo para funciones específicas de células y tejidos.

Principales métodos:

  • Análisis de datos de secuencia. análisis de datos de secuencia.
  • Análisis de los datos del microarray. análisis de los datos del microarray.
  • Revisión de la literatura científica más reciente.

Principales resultados:

  • El empalme alternativo contribuye significativamente a la diversidad proteómica en los metazoos.
  • Los esfuerzos en curso se centran en la identificación de las variantes de transcripción funcional y sus roles fisiológicos.
  • La investigación está explorando la coordinación global del empalme alternativo.

Conclusiones:

  • El empalme alternativo es crucial para la complejidad biológica en los metazoos.
  • Se necesita más investigación para caracterizar completamente las variantes de transcripción y sus redes reguladoras.