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Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
TIGAR, un regulador inducible por p53 de la glucólisis y la apoptosis
Karim Bensaad1, Atsushi Tsuruta, Mary A Selak
1The Beatson Institute for Cancer Research, Switchback Road, Glasgow G61 1BD, UK.
Cell
|July 15, 2006
Resumen
El supresor tumoral p53 regula la muerte celular a través del gen TIGAR. TIGAR reduce las especies reactivas de oxígeno (ROS) y la glucólisis, protegiendo a las células de la apoptosis inducida por p53 y el daño genómico.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Investigación del cáncer Investigación del cáncer.
- El metabolismo celular es el metabolismo celular.
Sus antecedentes:
- La proteína supresora de tumores p53 es crucial para prevenir el cáncer.
- Las funciones de p53 a través de la detención del ciclo celular, la apoptosis y el mantenimiento de la estabilidad del genoma.
Objetivo del estudio:
- Identificar nuevos genes inducibles al p53.
- Investigar el papel de TIGAR (regulador de la glucólisis y la apoptosis inducida por TP53) en las respuestas celulares mediadas por p53.
Principales métodos:
- Análisis de la expresión génica p53-inducible.
- Medición de los niveles de fructosa-2,6-bisfosfato y de las especies reactivas de oxígeno (ROS) intracelulares.
- Evaluación de la apoptosis celular y la sensibilidad a la muerte inducida por p53.
Principales resultados:
- Identificó TIGAR como un gen inducible por p53.
- La expresión de TIGAR disminuyó la fructosa-2,6-bisfosfato, inhibiendo la glicólisis y disminuyendo la ROS intracelular.
- TIGAR protegió a las células de la apoptosis asociada a ROS; TIGAR knockdown sensibilizó a las células a la muerte inducida por p53.
Conclusiones:
- TIGAR modula la respuesta apoptótica a p53, promoviendo la supervivencia celular bajo estrés.
- La reducción de las ROS intracelulares por TIGAR contribuye al papel de p53 en la prevención del daño genómico.
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