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Crossing Over01:34

Crossing Over

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Unlike mitosis, meiosis aims for genetic diversity in its creation of haploid gametes. Dividing germ cells first begin this process in prophase I, where each chromosome—replicated in S phase—is now composed of two sister chromatids (identical copies) joined centrally.
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process...
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Olfaction01:25

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The sense of smell is achieved through the activities of the olfactory system. It starts when an airborne odorant enters the nasal cavity and reaches olfactory epithelium (OE). The OE is protected by a thin layer of mucus, which also serves the purpose of dissolving more complex compounds into simpler chemical odorants. The size of the OE and the density of sensory neurons varies among species; in humans, the OE is only about 9-10 cm2.
The olfactory receptors are embedded in the cilia of the...
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Exon Recombination02:32

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The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
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Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I,...
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Olfactory Receptors: Location and Structure01:03

Olfactory Receptors: Location and Structure

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The process of olfaction, also known as the sense of smell, is a sophisticated chemical response system. The specialized sensory neurons that facilitate this process, known as olfactory receptor neurons, are situated in an upper segment of the nasal cavity, known as the olfactory epithelium. Olfactory sensory neurons are bipolar, with their dendrites extending from the epithelium's apex into the mucus that lines the nasal cavity. Airborne molecules, when inhaled, traverse the olfactory...
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Physiology of Smell and Olfactory Pathway01:20

Physiology of Smell and Olfactory Pathway

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Humans detect odors with the help of specialized cells located in the upper part of the nasal cavity, called olfactory receptor neurons (ORNs). ORNs possess hair-like structures called cilia, which are receptive to sensations from the inhaled air. When an odorant molecule binds to a specific receptor on the cell of the cilia, it leads to a series of events that ultimately cause the ORN to send electrical signals to the olfactory bulb in the brain through the olfactory nerves.
The olfactory...
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Updated: Apr 28, 2026

High-throughput Analysis of Mammalian Olfactory Receptors: Measurement of Receptor Activation via Luciferase Activity
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High-throughput Analysis of Mammalian Olfactory Receptors: Measurement of Receptor Activation via Luciferase Activity

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Interacciones intercromosómicas y elección del receptor olfativo.

Stavros Lomvardas1, Gilad Barnea, David J Pisapia

  • 1Department of Biochemistry and Molecular Biophysics and Howard Hughes Medical Institute, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.

Cell
|July 29, 2006
PubMed
Resumen

Un solo elemento potenciador (H) en el cromosoma 14 se asocia con los promotores del gen del receptor olfativo (OR). Este potenciador puede controlar la activación estocástica de un solo gen OR en las neuronas sensoriales.

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Área de la Ciencia:

  • Biología del sistema olfativo Biología del sistema olfativo
  • Genética y genómica.
  • Mecanismos moleculares de la regulación génica.

Sus antecedentes:

  • La expresión génica del receptor olfativo (OR) en las neuronas sensoriales individuales es crucial para la organización y función del sistema olfativo.
  • Los OR forman una gran familia de genes, y por lo general sólo un gen OR se expresa por neurona.
  • La comprensión de los mecanismos reguladores que rigen la expresión génica de una sola OR es clave para la investigación olfativa.

Objetivo del estudio:

  • Investigar el papel de los elementos potenciadores en la regulación de la expresión génica de los receptores olfativos.
  • Para determinar cómo un elemento potenciador específico (H) interactúa con los promotores del gen OR.
  • Aclarar el mecanismo que controla la elección de un solo gen OR para su expresión en las neuronas sensoriales olfativas.

Principales métodos:

  • La captura de la conformación cromosómica (3C) se empleó para identificar las interacciones entre los elementos potenciadores y los promotores del gen OR.
  • La fluorescencia de ADN y ARN en hibridación in situ (FISH) se utilizó para visualizar la asociación física y el estado transcripcional de las interacciones potenciador-promotor.
  • Se crearon modelos de ratones transgénicos para estudiar los efectos de elementos potenciadores adicionales en la expresión de OR.

Principales resultados:

  • Se demostró que el elemento potenciador H en el cromosoma 14 se asocia específicamente con los promotores de genes OR ubicados en diferentes cromosomas.
  • Los experimentos FISH confirmaron la colocalización del potenciador H con el único alelo OR transcrito activamente en las neuronas sensoriales.
  • Los ratones transgénicos con elementos H adicionales exhibieron expresión de un segundo receptor funcional en las neuronas que normalmente expresan pseudogenes OR.

Conclusiones:

  • Un solo elemento potenciador de acción trans (H) puede mediar la asociación específica con múltiples promotores de genes OR.
  • El potenciador H juega un papel crítico en la activación estocástica de un solo alelo OR en las neuronas sensoriales olfativas.
  • Estos hallazgos proponen un modelo en el que un único potenciador regula la elección del gen del receptor olfativo.