Search research articles

ACERCA DE JoVE

Visión General Liderazgo Blog Centro de Ayuda JoVE

AUTORES

Proceso de Publicación Consejo Editorial Alcance y Políticas Revisión por Pares Preguntas Frecuentes Enviar

BIBLIOTECARIOS

Testimonios Suscripciones Acceso Recursos Consejo Asesor de Bibliotecas Preguntas Frecuentes

INVESTIGACIÓN

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archivo

EDUCACIÓN

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Centro de Recursos para Profesores Sitio de Profesores

Términos y Condiciones de Uso

Política de Privacidad

Videos de Conceptos Relacionados

Mass Spectrometry: Aldehyde and Ketone Fragmentation

Mass Spectrometry: Aldehyde and Ketone Fragmentation

In mass spectrometry, the fragmentation of aliphatic aldehydes and ketones generally occurs through three key mechanisms: α-cleavage, inductive cleavage, and the McLafferty rearrangement.

MALDI-TOF Mass Spectrometry

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...

Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

High-Performance Liquid Chromatography: Types of Detectors

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por

Same author

Rotating hinge expandable prostheses may have a higher risk of medial tibial plateau deformity compared to constrained hinged expandable prostheses following reconstruction of distal femur in children with primary bone tumors.

European journal of orthopaedic surgery & traumatology : orthopedie traumatologie·2026

Same author

Bioorthogonal chemical labeling of endogenous receptors in the live mouse brain.

Methods in enzymology·2026

Same author

Single-molecule kinetic exploration of functional sub-states in an evolving phosphotriesterase.

Nature communications·2026

Same author

In-house three dimensional-printed cutting guides improve surgical accuracy in children who underwent resection of malignant bone tumours of lower limb and reconstruction with allograft.

International orthopaedics·2026

Same author

A press-fit tibial smooth stem may preserve proximal tibial physeal growth after expandable prosthesis reconstruction following resection of distal femur in children with bone sarcoma.

European journal of orthopaedic surgery & traumatology : orthopedie traumatologie·2026

Same author

Construction of a CO<sub>2</sub>-Fixing Compartment Using a Shape-Transforming DNA Scaffold.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026

Search research articles

Video Experimental Relacionado

Updated: Jul 20, 2026

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

Una matriz de lectina fluorescente que utiliza hidrogel supramolecular para una simple detección y perfilado de

Yoichiro Koshi¹, Eiji Nakata, Hiroki Yamane

¹PRESTO (Synthesis and Control, JST), Department of Synthetic Chemistry and Biological Chemistry, Kyoto University, Kyotodaigaku-Katsura, Nishikyo-ku, Kyoto, 615-8510, Japan.

Journal of the American Chemical Society

|August 10, 2006

Resumen

Una nueva matriz de lectina fluorescente en una matriz de hidrogel permite un análisis rápido y de alto rendimiento de los glucoconjugados. Este método permite la detección de sacarídeos sensibles sin etiquetado ni lavado, simplificando el análisis de muestras biológicas complejas.

Más Videos Relacionados

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Videos de Experimentos Relacionados

Last Updated: Jul 20, 2026

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Área de la Ciencia:

La bioquímica es la bioquímica.
Química analítica Química analítica es la que
Ciencia de los materiales Ciencia de los materiales.

Sus antecedentes:

Los glicoconjugados son cruciales en los procesos biológicos, lo que requiere métodos analíticos eficientes.
Las técnicas existentes para el análisis de sacarídeos pueden ser complejas y consumir mucho tiempo.

Objetivo del estudio:

Desarrollar una nueva matriz de lectina fluorescente para la detección de sacarídeos de alto rendimiento.
Para utilizar una matriz de hidrogel supramolecular para mejorar la estabilidad y la función de la lectina.

Principales métodos:

Construcción de una matriz de lectina fluorescente dentro de un hidrogel supramolecular.
Inmovilización no covalente de lectinas fluorescentes bajo condiciones semi-húmedas.
Aplicación del apagado y recuperación de fluorescencia bimolecular (BFQR) para la detección de sacarídeos.

Principales resultados:

Las lectinas inmovilizadas conservaron capacidades de reconocimiento molecular dentro de la matriz de hidrogel.
Lectura fluorescente exitosa de varios sacáridos, incluida la glucosa, los oligosacáridos y las glicoproteínas.
Se ha demostrado la detección de alto rendimiento y la elaboración de perfiles de muestras complejas como lisatos celulares.

Conclusiones:

La matriz de lectina desarrollada ofrece una plataforma sensible y eficiente para el análisis de glucoconjugados.
La matriz de hidrogel preserva eficazmente la función de la lectina, lo que permite una detección robusta de los sacarídeos.
Este método simplifica el análisis al eliminar la necesidad de etiquetar el objetivo y los pasos de lavado.