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Estructura de la solución de una horquilla de ARN inusualmente estable, 5'GGAC(UUCG)GUCCCC

C Cheong¹, G Varani, I Tinoco

¹Department of Chemistry, University of California, Berkeley 94720.

|August 26, 1990

Resumen

Este estudio revela la estructura estable de la horquilla del ARN, 5'GGAC(UUCG) GUCC, utilizando RMN. Los hallazgos explican su estabilidad e interacción con las proteínas, cruciales para el plegamiento del ARN.

Área de la Ciencia:

Biología Molecular Biología Molecular
Biología Estructural Biología estructural.
La bioquímica es la bioquímica.

Sus antecedentes:

Los bucles de horquilla de ARN son vitales para la estructura y función del ARN.
Existe un conocimiento limitado con respecto a las conformaciones de la horquilla del ARN.
Los datos existentes provienen principalmente de las estructuras cristalinas de ARN de transferencia y los estudios de horquilla de ADN.

Objetivo del estudio:

Para determinar la estructura de alta resolución de una horquilla de ARN común y estable: 5'GGAC(UUCG)GUCC.
Para aclarar la base estructural de la inusual estabilidad de la horquilla.
Comprender sus implicaciones para el plegamiento del ARN, las interacciones de las proteínas y la transcripción enzimática.

Principales métodos:

Se empleó la espectroscopia de resonancia magnética nuclear (RMN).
Se midieron las distancias interprotónicas y las constantes de acoplamiento escalar.
Se obtuvo un modelo estructural de alta resolución de la horquilla de ARN.

Principales resultados:

El bucle de la horquilla se estabiliza mediante un par de bases G.U y un extenso apilamiento de bases.
La guanina se observó en la conformación sin.
Un contacto citosina-fosfato contribuye a la estabilidad del bucle.

Conclusiones:

La estructura determinada explica la estabilidad excepcional de la horquilla de ARN 5'GGAC ((UUCG) GUCC.
Las características estructurales sugieren roles en la nucleación del pliegue del ARN y la unión a las proteínas.
La estructura de la horquilla impide la progresión de la transcriptasa inversa, a diferencia de otras estructuras secundarias de ARN.

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