Video Experimental Relacionado

Updated: Jul 15, 2026

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Published on: November 2, 2013

Una búsqueda de ribozimas en todo el genoma revela una secuencia similar al HDV en el gen CPEB3 humano

Kourosh Salehi-Ashtiani¹, Andrej Lupták, Alexander Litovchick

¹Howard Hughes Medical Institute, Department of Molecular Biology, and Center for Computational and Integrative Biology (CCIB), 7215 Simches Research Center, Massachusetts General Hospital, 185 Cambridge Street, Boston, MA 02114, USA.

Science (New York, N.Y.)

|September 23, 2006

Resumen

Los científicos descubrieron una nueva ribozima en el genoma humano, una molécula de ARN autoclasificante dentro del gen CPEB3. Este hallazgo arroja luz sobre la evolución de las enzimas de ARN y sus posibles orígenes en el transcriptoma humano.

Área de la Ciencia:

Biología Molecular Biología Molecular
Biología evolutiva Biología evolutiva.
La genómica es la genómica.

Sus antecedentes:

Las ribozimas son moléculas catalíticas de ARN cruciales para la vida temprana.
Se han identificado pocos ribozimas en los organismos modernos.
La comprensión de la evolución de las ribozimas proporciona información sobre los orígenes de la vida.

Objetivo del estudio:

Para aislar ARN autoclasificados del genoma humano utilizando la selección in vitro.
Para caracterizar las ribozimas recién descubiertas y su importancia evolutiva.
Investigar la relación entre los ribozimas de mamíferos y los ribozimas virales conocidos.

Principales métodos:

Se emplearon técnicas de selección in vitro para examinar el genoma humano.
Se aislaron secuencias de ARN que exhibían actividad de auto-escisión.
Se realizaron análisis estructurales y bioquímicos de las ribozimas identificadas.
El análisis filogenético se utilizó para determinar los orígenes evolutivos.

Principales resultados:

Se aislaron con éxito varios ribozimas autoclavantes del genoma humano.
Se identificó una ribozima mamífera conservada dentro de un intrón del gen CPEB3.
La ribozima CPEB3 muestra similitudes estructurales y bioquímicas con las ribozimas del virus de la hepatitis delta (VHD).
La presencia exclusiva de esta ribozima en los mamíferos sugiere un origen evolutivo reciente (aprox. hace 200 millones de años).

Conclusiones:

El descubrimiento de la ribozima CPEB3 amplía el repertorio conocido de ribozimas funcionales en mamíferos.
Este hallazgo apoya la hipótesis de que los ribozimas del HDV pueden haberse originado en el transcriptoma humano.
El estudio proporciona evidencia de la evolución en curso de los ARN catalíticos dentro del genoma humano.

Más Videos Relacionados

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

Published on: July 21, 2014

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

Videos de Conceptos Relacionados

Complementary DNA

Complementary DNA

Ribozymes

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...

Multi-species Conserved Sequences

Multi-species Conserved Sequences

Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...

Complementary DNA

Complementary DNA

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

Ribozymes

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...