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Renjun Pei¹, Steven K Taylor, Darko Stefanovic

¹NSF Chemical Bonding Center: Center for Molecular Cybernetics, Division of Experimental Therapeutics, Department of Medicine, Columbia University, New York, NY 10032, USA.

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|September 28, 2006

Resumen

Hemos creado nuevos conjuntos policatalíticos utilizando catalizadores de ácido nucleico (deoxirribozimas) y estreptavidina. Estos conjuntos dividen de manera eficiente sustratos en una matriz, ofreciendo movimiento y actividad controlados para aplicaciones potenciales.

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Área de la Ciencia:

La bioquímica es la bioquímica.
Biología Molecular Biología Molecular
Nanotecnología La nanotecnología es la nanotecnología.

Sus antecedentes:

Los catalizadores de ácido nucleico, o desoxirribozimas, ofrecen una actividad enzimática programable.
El desarrollo de sistemas catalíticos eficientes que imiten procesos biológicos es un desafío clave.

Objetivo del estudio:

Para diseñar y caracterizar conjuntos policatalizantes con actividad de fosfodiesterasa.
Para investigar el movimiento controlado y la eficiencia catalítica de estos conjuntos en una matriz rica en sustrato.

Principales métodos:

Construcción de conjuntos policatalíticos con números variables de desoxirribozimas y unidades de estreptavidina.
Caracterización a nivel de conjunto utilizando resonancia plasmónica de superficie (SPR).
Observación de la actividad catalítica en matrices recubiertas de oligonucleótidos.

Principales resultados:

Los ensamblajes policatalíticos demostraron una escisión continua del sustrato en una matriz de alta densidad.
Las tasas de escisión fueron comparables a las de los desoxirribozimas individuales en solución.
La difusión del ensamblaje, la procesividad y los tiempos de residencia eran sintonizables alterando el número de unidades catalíticas y las secuencias de reconocimiento.

Conclusiones:

Los conjuntos policatalíticos representan una nueva plataforma para la actividad enzimática controlada.
La capacidad de ajustar la dinámica de ensamblaje abre posibilidades para sofisticadas máquinas moleculares.
Este trabajo avanza en el campo de los catalizadores de ácido nucleico de ingeniería.