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La absorción dependiente del sodio del fosfato inorgánico por el parásito de la malaria intracelular
Kevin J Saliba1, Rowena E Martin, Angelika Bröer
1School of Biochemistry and Molecular Biology, The Australian National University, Canberra ACT 0200, Australia.
Nature
|September 29, 2006
Resumen
Los parásitos de la malaria utilizan el gradiente de sodio (Na+) a través de su membrana para absorber el fosfato inorgánico esencial (P(i)). Este descubrimiento revela un nuevo mecanismo para la absorción de nutrientes en Plasmodium falciparum.
Área de la Ciencia:
- Parasitología Parasitología.
- Biología Molecular Biología Molecular
- La bioquímica es la bioquímica.
Sus antecedentes:
- La infección por el parásito de la malaria (Plasmodium falciparum) aumenta la permeabilidad de los eritrocitos del huésped a solutos como Na+ y K+.
- Esto conduce a una mayor concentración de Na+ en el citosol de los eritrocitos y a un fuerte gradiente de Na+ hacia adentro a través de la membrana del parásito.
Objetivo del estudio:
- Para investigar cómo el Plasmodium falciparum utiliza el gradiente Na+ para la absorción de nutrientes.
- Para identificar y caracterizar el transportador responsable de la absorción de fosfato inorgánico (P)
Principales métodos:
- Se estudió la absorción de fosfato inorgánico (P) en parásitos intracelulares.
- Clonado y caracterizado el transportador de P (PfPiT) de Plasmodium falciparum.
- PfPiT expresado en ovocitos de Xenopus para confirmar la función.
Principales resultados:
- Plasmodium falciparum absorbe el fosfato inorgánico (P) a través de un transportador dependiente de Na+.
- El transportador exhibe una estequiometría de 2Na+:1P(i) y prefiere la P(i) monovalente.
- El transportador clonado de PfPiT, expresado en ovocitos, medió la absorción de P (i) dependiente de Na+ con características similares.
Conclusiones:
- El parásito de la malaria explota el entorno iónico alterado de la célula huésped para energizar la absorción de fosfato inorgánico (P).
- PfPiT es un transportador de superficie de parásito clave involucrado en la adquisición de nutrientes.
- Esto destaca la adaptación del parásito para manipular la fisiología de la célula huésped para su crecimiento.
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