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Asociación del receptor del antígeno de las células B con la proteína tirosina quinasa Lyn Lyn
Y Yamanashi1, T Kakiuchi, J Mizuguchi
1Department of Oncology, University of Tokyo, Japan.
Science (New York, N.Y.)
|January 11, 1991
Resumen
La unión del antígeno a las células B desencadena la señalización. La proteína tirosina quinasa de Lyn se asocia con las IgM de la superficie de las células B, lo que sugiere su papel en el crecimiento y la diferenciación celular mediada por antígenos.
Área de la Ciencia:
- Inmunología Inmunología.
- La señalización celular de las células.
- La bioquímica es la bioquímica.
Sus antecedentes:
- La activación de las células B implica la unión cruzada de antígenos con las inmunoglobulinas ligadas a la membrana (Igs).
- Las proteínas tirosina quinasas son cruciales para la proliferación de células B y la señalización de la diferenciación.
- La tirosina quinasa de tipo Src Lyn se expresa predominantemente en las células B.
Objetivo del estudio:
- Investigar la asociación de Lyn con las IgM ligadas a la membrana.
- Para determinar el papel de Lyn en la transducción de señales mediada por antígenos en las células B.
Principales métodos:
- Coinmunoprecipitación de la proteína Lyn y su actividad quinasa con IgM de lisatos celulares detergentes.
- Análisis de los patrones de fosforilación de la tirosina en las células B tras el enlace cruzado de IgM ligado a la membrana.
Principales resultados:
- La proteína Lyn y su actividad quinasa fueron coinmunoprecipitadas con IgM.
- El enlace cruzado de IgM ligado a la membrana aumentó rápidamente la fosforilación de tirosina de múltiples proteínas de células B.
- Lyn está físicamente asociado con las IgM ligadas a la membrana.
Conclusiones:
- Lyn está físicamente asociado con las IgM ligadas a la membrana.
- La linquinasa está implicada en la vía de transducción de la señal iniciada por la unión del antígeno a las células B.
- Esta asociación sugiere la participación de Lyn en la proliferación y diferenciación de células B mediadas por antígenos.
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