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Dos modos de apertura de los poros de fusión revelados por las grabaciones de las células en una sinapsis
Liming He1, Xin-Sheng Wu, Raja Mohan
1National Institute of Neurological Disorders and Stroke, 35 Convent Drive, Building 35, Room 2B-1012, Bethesda, Maryland 20892, USA.
Nature
|October 27, 2006
Resumen
La fusión de beso y huida, un mecanismo controvertido, ocurre en las sinapsis y puede generar rápidas corrientes postsinápticas. Los diferentes tamaños de los poros de fusión controlan la cinética y la amplitud de la corriente sináptica.
Área de la Ciencia:
- La neurociencia es la neurociencia.
- Biología celular Biología celular.
- La biofísica es la biofísica.
Sus antecedentes:
- La fusión de la vesícula con la membrana celular libera neurotransmisores.
- El tamaño del poro de fusión influye en la velocidad de liberación del transmisor.
- Se debate la fusión de "besar y huir" y su papel en la transmisión sináptica.
Objetivo del estudio:
- Investigar la cinética de los poros de fusión durante la fusión de una sola vesícula en las sinapsis.
- Determinar si la fusión "besar y huir" se produce en las sinapsis de tipo cáliz.
- Evaluar la contribución de diferentes tamaños de poros de fusión a las corrientes sinápticas.
Principales métodos:
- Registrar la cinética de los poros de fusión durante la fusión de una sola vesícula.
- Analizando la duración del parpadeo de la capacitancia y la conductividad de los poros de fusión (G(p)).
- Diferenciar entre el colapso total y los eventos de fusión de "besar y huir".
Principales resultados:
- Tanto el colapso completo como la fusión de "besar y huir" se observaron en sinapsis de tipo cáliz.
- El colapso completo mostró un alto G inicial (p) (>375 pS) con un rápido aumento.
- La fusión de "besar y huir" exhibió breves parpadeos de capacitancia (<2 s) con G ((p) variando de 15-288 pS a >288 pS.
Conclusiones:
- Se ha confirmado que la fusión de "besar y huir" ocurre en las sinapsis.
- Las variaciones en el tamaño de los poros de fusión, incluso en "besar y huir", pueden generar rápidas corrientes postsinápticas.
- Diversos tamaños de poros de fusión regulan la cinética y la amplitud de la corriente sináptica.
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