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Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
La inactivación de la vía p53 en el retinoblastoma
Nikia A Laurie1, Stacy L Donovan, Chie-Schin Shih
1Department of Developmental Neurobiology, St Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.
Nature
|November 3, 2006
Resumen
Los tumores de retinoblastoma no surgen de células resistentes a la muerte. En cambio, la vía p53 es suprimida por la amplificación de MDMX, lo que hace que MDMX sea un objetivo terapéutico potencial para este cáncer de ojo.
Área de la Ciencia:
- Oncología Oncología.
- Biología Molecular Biología Molecular
- Biología del desarrollo Biología del desarrollo.
Sus antecedentes:
- El retinoblastoma (RB) es un cáncer ocular infantil.
- La RB es iniciada por mutaciones en el gen del retinoblastoma 1 (RB1).
- La teoría predominante sugiere que la RB surge de células intrínsecamente resistentes a la muerte, evitando la vía p53.
Objetivo del estudio:
- Investigar el papel de la vía p53 en el desarrollo del retinoblastoma.
- Para determinar si las células deficientes de RB1 son intrínsecamente resistentes a la muerte.
- Para identificar posibles dianas terapéuticas para el retinoblastoma.
Principales métodos:
- Estudió la vía de vigilancia tumoral (Arf, MDM2, MDMX, p53) en retinoblastos con deficiencia de RB1.
- Se analizó la apoptosis mediada por p53 y la salida del ciclo celular.
- Examinó la amplificación del gen MDMX y la expresión de proteínas durante la progresión tumoral.
Principales resultados:
- Los retinoblastos deficientes de RB1 activan la vía p53, sufren apoptosis y salen del ciclo celular.
- La amplificación del gen MDMX y el aumento de la expresión de proteínas durante la progresión tumoral.
- Esta regulación ascendente de MDMX suprime la respuesta p53 en las células deficientes de RB1.
Conclusiones:
- La vía p53 está inactivada en el retinoblastoma, contrariamente a la teoría intrínseca de la resistencia a la muerte.
- El retinoblastoma no se origina en células intrínsecamente resistentes a la muerte.
- MDMX es un objetivo quimioterapéutico específico y prometedor para el tratamiento del retinoblastoma.
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