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Un disparador de dos golpes para la biogénesis de siRNA en las plantas.
Michael J Axtell1, Calvin Jan, Ramya Rajagopalan
1Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
Cell
|November 4, 2006
Resumen
Los sitios de unión duales de microARN, que no siempre implican la escisión, desencadenan la biogénesis de ARN pequeño interferente (siRNA) en plantas. Este mecanismo conservado tiene un impacto en el silenciamiento y el reconocimiento del ARN.
Área de la Ciencia:
- Biología molecular vegetal Biología molecular vegetal
- Las vías de silenciamiento de ARN son vías de silenciamiento de ARN.
- Epigenética y regulación génica.
Sus antecedentes:
- Los microARN (miRNA) inician la escisión dirigida por el ARN, lo que a veces conduce a pequeños ARN interferentes (siRNA) por fases en Arabidopsis.
- La mayoría de las dianas de miRNA no producen siRNA, lo que indica que otros factores están involucrados en la generación de siRNA.
Objetivo del estudio:
- Investigar los determinantes de la biogénesis del siRNA en fases más allá de la simple escisión del miRNA.
- Explorar el papel de los sitios de unión dual de miRNA en los loci de siRNA conservados.
Principales métodos:
- Análisis comparativo de la generación de siRNA en musgo y Arabidopsis.
- Ensayos de unión in vitro para miR390 y sitios complementarios.
- Análisis funcional de los sitios complementarios miR390 en Arabidopsis utilizando enfoques genéticos.
Principales resultados:
- Los siRNA de fase en el musgo se originan en regiones flanqueadas por sitios de escisión duales de miR390.
- El locus siRNA conservado AtTAS3 posee sitios complementarios duales miR390, ambos funcionales en Arabidopsis.
- Los sitios de unión de miRNA no clivantes pueden ser funcionalmente significativos en las plantas.
- Los sitios complementarios duales miRNA / siRNA, no necesariamente dos eventos de escisión, son un desencadenante conservado para la biogénesis del siRNA.
Conclusiones:
- Un mecanismo conservado que involucra sitios de unión dual de miRNA, a menudo pero no exclusivamente a través de la escisión, inicia la producción de siRNA por fases.
- Este hallazgo tiene implicaciones significativas para la comprensión del reconocimiento de ARN y el silenciamiento de ARN aberrantes en todas las especies de plantas.
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