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trkB codifica un receptor funcional para el factor neurotrófico derivado del cerebro y la neurotropina-3, pero no

Stephen P Squinto¹, Trevor N Stitt, Thomas H Aldrich

¹Regeneron Pharmaceuticals, Inc. 777 Old Saw Mill River Road Tarrytown, New York 10591, USA.

|May 31, 1991

Resumen

El factor neurotrófico derivado del cerebro (BDNF) y la neurotropina-3 (NT-3) se unen al receptor trkB, a diferencia del factor de crecimiento nervioso (NGF). Este descubrimiento identifica trkB como un componente clave del receptor funcional para BDNF y NT-3.

Área de la Ciencia:

La neurociencia es la neurociencia.
Biología Molecular Biología Molecular
Biología celular Biología celular.

Sus antecedentes:

El factor neurotrófico derivado del cerebro (BDNF) y la neurotropina-3 (NT-3) están implicados en la supervivencia neuronal.
El factor de crecimiento nervioso (NGF) es otro miembro de la familia de las neurotrofinas con funciones distintas.
Los receptores específicos que median las acciones de BDNF y NT-3 no fueron completamente esclarecidos.

Objetivo del estudio:

Para investigar las interacciones de los receptores de BDNF, NT-3 y NGF.
Para identificar el receptor funcional para BDNF y NT-3.
Para diferenciar las vías de señalización de estas neurotrofinas.

Principales métodos:

Ensayos vinculantes para evaluar la interacción de las neurotrofinas con el receptor NGF de baja afinidad y trkB.
Ensayos funcionales utilizando células PC12 que expresan trkB para evaluar las respuestas mediadas por neurotrofinas.
Comparación de las interacciones de BDNF, NT-3 y NGF con las receptoras tirosina quinasas.

Principales resultados:

BDNF, NT-3 y NGF se unen al receptor de NGF de baja afinidad.
BDNF y NT-3, pero no NGF, se unen al receptor tirosina quinasa trkB.
trkB media las respuestas funcionales al BDNF y al NT-3 en las células PC12, siendo el BDNF un ligando más potente.

Conclusiones:

trkB se identifica como un componente receptor funcional para BDNF y NT-3, pero no para NGF.
Esta investigación aclara el uso distinto de los receptores de las neurotropinas.
Los hallazgos sugieren la existencia de receptores adicionales de neurotrofinas.

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