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YY1 es una proteína de unión de secuencia iniciadora que dirige y activa la transcripción in vitro
1Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, New Jersey 08544-1014.
Nature
|November 21, 1991
Resumen
El factor de transcripción YY1 se une a la región +1 del promotor P5 del virus tipo 2 adeno-asociado, regulando la iniciación de la transcripción. Esta unión es esencial para la transcripción basal e involucra factores relacionados con YY1 en otros genes.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Regulación genética Reglamento genético.
- La bioquímica es la bioquímica.
Sus antecedentes:
- La transcripción del ARNm eucariota está regulada por los elementos de la secuencia de ADN y los factores de transcripción.
- Los elementos clave incluyen regiones promotor-proximales, potenciadores, represores y silenciadores.
- Si bien los factores de unión a TATA están bien estudiados, los factores en la región de iniciación +1 son menos conocidos.
Objetivo del estudio:
- Investigar el papel de la región +1 del promotor P5 del virus adeno-asociado tipo 2 en la transcripción.
- Para identificar los factores de transcripción que interactúan con el elemento P5 + 1.
- Para determinar la necesidad y suficiencia del elemento P5 +1 para la transcripción basal.
Principales métodos:
- Ensayo de desplazamiento de movilidad electroforética (EMSA) para identificar factores de unión al ADN.
- Experimentos de agotamiento y reconstitución utilizando extractos de HeLa y Drosophila.
- Ensayos funcionales utilizando el factor de transcripción YY1 (YY1) y anticuerpos específicos de YY1.
Principales resultados:
- El elemento P5 +1 se une al factor de transcripción YY1.1.
- Este elemento es a la vez necesario y suficiente para una transcripción basal precisa.
- El YY1 parcialmente purificado restauró la transcripción en extractos empobrecidos de YY1, y los anticuerpos YY1 bloquearon la reactivación.
- Se identificaron factores relacionados con YY1 que se unen a los iniciadores de transcripción en otros genes celulares.
Conclusiones:
- El factor de transcripción YY1 juega un papel crucial en la regulación de la iniciación de la transcripción en el elemento P5 + 1.
- El elemento P5 +1 es una región reguladora clave para la transcripción basal.
- YY1 y los factores relacionados están involucrados en iniciar la transcripción a través de varios genes.
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