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Analysis of Histone Antibody Specificity with Peptide Microarrays
Published on: August 1, 2017
Rtt109 acetila la histona H3 lisina 56 y las funciones en la replicación del ADN
Junhong Han1, Hui Zhou, Bruce Horazdovsky
1Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA.
Science (New York, N.Y.)
|February 3, 2007
Resumen
El producto 109 del gen de transposición Ty1 (Rtt109) se identifica como una histona acetiltransferasa (HAT) responsable de la acetilación de H3-K56. La pérdida de Rtt109 conduce a la sensibilidad al daño del ADN y la inestabilidad cromosómica.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- La epigenética es la epigenética.
- Biología de la cromatina Biología de la cromatina
Sus antecedentes:
- La acetilación de histonas regula la expresión génica y la reparación del ADN.
- La acetilación de la histona H3 lisina 56 (H3-K56) es crucial para la respuesta al daño del ADN.
- La histona acetiltransferasa (HAT) específica para H3-K56 no fue identificada.
Objetivo del estudio:
- Para identificar el HAT responsable de la acetilación global H3-K56.
- Investigar el papel funcional de la acetilación H3-K56 en el mantenimiento de la estabilidad del genoma.
Principales métodos:
- Análisis genético de los mutantes de Rtt109.
- Análisis bioquímicos para determinar la actividad de HAT.
- Evaluación de la sensibilidad al daño del ADN y la ruptura cromosómica.
Principales resultados:
- Rtt109 fue identificado como el HAT que cataliza la acetilación H3-K56.
- La deficiencia o mutación de Rtt109 resultó en la pérdida de la acetilación H3-K56.
- Las células que carecían de Rtt109 mostraron una mayor sensibilidad a los agentes genotóxicos y elevadas rupturas cromosómicas espontáneas.
Conclusiones:
- Rtt109 es un HAT único esencial para la acetilación de H3-K56.
- La acetilación H3-K56 mediada por Rtt109 juega un papel crítico en la estabilidad del genoma y la reparación de daños en el ADN.
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